Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 71122..71669 Replicon plasmid tig00003056
Accession NZ_CP021681
Organism Escherichia coli strain AR_0162

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag AM408_RS00520 Protein ID WP_000969996.1
Coordinates 71388..71669 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag AM408_RS00515 Protein ID WP_021546940.1
Coordinates 71122..71391 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM408_RS00480 66827..67411 - 585 WP_000645940.1 DUF1788 domain-containing protein -
AM408_RS00485 67408..68007 - 600 WP_001021938.1 DUF1819 family protein -
AM408_RS00490 68017..68943 - 927 WP_000617209.1 WYL domain-containing protein -
AM408_RS26320 69012..69329 - 318 WP_032156934.1 hypothetical protein -
AM408_RS26325 69553..69732 + 180 WP_012600012.1 hypothetical protein -
AM408_RS00500 69764..70375 - 612 Protein_71 IS3 family transposase -
AM408_RS00515 71122..71391 + 270 WP_021546940.1 hypothetical protein Antitoxin
AM408_RS00520 71388..71669 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM408_RS00525 71715..71927 + 213 Protein_74 3'-5' exonuclease -
AM408_RS00530 72001..72210 + 210 Protein_75 transposase -
AM408_RS00535 72511..74061 - 1551 Protein_76 Tn3-like element TnAs1 family transposase -
AM408_RS00545 74116..74820 - 705 WP_001067852.1 IS6-like element IS26 family transposase -
AM408_RS00550 75166..75975 + 810 WP_000161640.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / qnrS1 / blaCTX-M-15 - 1..95850 95850
- inside IScluster/Tn - - 69764..74820 5056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T76930 WP_000969996.1 NZ_CP021681:71388-71669 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T76930 NZ_CP021681:71388-71669 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT76930 WP_021546940.1 NZ_CP021681:71122-71391 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT76930 NZ_CP021681:71122-71391 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGTGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGCGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References