Detailed information of TA system TA076929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1470286..1471205
• Replicon: chromosome
• Accession: NZ_CP021677
• Organism: Bacillus licheniformis strain SRCM100027

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: S100027_RS07675
• Protein ID: WP_003180879.1
• Coordinates: 1470459..1471205 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: S100027_RS07670
• Protein ID: WP_003180877.1
• Coordinates: 1470286..1470459 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S100027_RS07650	1465711..1466808	+	1098	WP_003180867.1	mannonate dehydratase	-
S100027_RS07655	1466784..1467632	+	849	WP_003180869.1	SDR family oxidoreductase	-
S100027_RS07660	1467659..1468672	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
S100027_RS07665	1468725..1469996	+	1272	WP_009328725.1	MFS transporter	-
S100027_RS07670	1470286..1470459	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
S100027_RS07675	1470459..1471205	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
S100027_RS07680	1471316..1472314	-	999	WP_009328723.1	inorganic phosphate transporter	-
S100027_RS07685	1472327..1472944	-	618	WP_003180884.1	DUF47 domain-containing protein	-
S100027_RS07690	1473276..1475033	+	1758	WP_011197813.1	gamma-glutamyltransferase	-
S100027_RS07695	1475162..1475805	+	644	Protein_1439	DUF624 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T76929 WP_003180879.1 NZ_CP021677:c1471205-1470459 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T76929 NZ_CP021677:c1471205-1470459 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT76929 WP_003180877.1 NZ_CP021677:c1470459-1470286 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT76929 NZ_CP021677:c1470459-1470286 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37