Detailed information of TA system TA076928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 559107..559743
• Replicon: chromosome
• Accession: NZ_CP021677
• Organism: Bacillus licheniformis strain SRCM100027

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: S100027_RS02785
• Protein ID: WP_003179128.1
• Coordinates: 559393..559743 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: S100027_RS02780
• Protein ID: WP_006638778.1
• Coordinates: 559107..559388 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S100027_RS02760	554222..555703	+	1482	WP_009330132.1	PH domain-containing protein	-
S100027_RS02765	555700..556299	-	600	WP_003179118.1	rhomboid family intramembrane serine protease	-
S100027_RS02770	556642..557601	+	960	WP_152847888.1	outer membrane lipoprotein carrier protein LolA	-
S100027_RS02775	557826..558995	+	1170	WP_026080763.1	alanine racemase	-
S100027_RS02780	559107..559388	+	282	WP_006638778.1	hypothetical protein	Antitoxin
S100027_RS02785	559393..559743	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
S100027_RS02790	559861..560688	+	828	WP_061578564.1	RsbT co-antagonist protein RsbRA	-
S100027_RS02795	560692..561057	+	366	WP_003179132.1	STAS domain-containing protein	-
S100027_RS02800	561060..561461	+	402	WP_003179135.1	anti-sigma regulatory factor	-
S100027_RS02805	561472..562479	+	1008	WP_003179137.1	PP2C family protein-serine/threonine phosphatase	-
S100027_RS02810	562538..562864	+	327	WP_003179140.1	anti-sigma factor antagonist	-
S100027_RS02815	562864..563349	+	486	WP_003179142.1	anti-sigma B factor RsbW	-
S100027_RS02820	563315..564106	+	792	WP_003179144.1	RNA polymerase sigma factor SigB	-
S100027_RS02825	564103..564702	+	600	WP_003179145.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T76928 WP_003179128.1 NZ_CP021677:559393-559743 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T76928 NZ_CP021677:559393-559743 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT76928 WP_006638778.1 NZ_CP021677:559107-559388 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT76928 NZ_CP021677:559107-559388 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2