Detailed information of TA system TA076925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 48007..48572
• Replicon: plasmid p5D_Fsymbiotica-3
• Accession: NZ_CP021662
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCS41_RS15345
• Protein ID: WP_119798012.1
• Coordinates: 48007..48312 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CCS41_RS15350
• Protein ID: WP_119798013.1
• Coordinates: 48300..48572 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS15325	44085..44645	+	561	WP_119797797.1	conjugal transfer protein TraX	-
CCS41_RS15330	44716..44868	-	153	WP_119797796.1	Hok/Gef family protein	-
CCS41_RS15335	45116..47263	+	2148	WP_119798010.1	DotA/TraY family protein	-
CCS41_RS15340	47343..47981	+	639	WP_119798011.1	plasmid IncI1-type surface exclusion protein ExcA	-
CCS41_RS15345	48007..48312	-	306	WP_119798012.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCS41_RS15350	48300..48572	-	273	WP_119798013.1	CopG family ribbon-helix-helix protein	Antitoxin
CCS41_RS15355	48647..49753	+	1107	Protein_53	conjugal transfer protein TrbA	-
CCS41_RS15360	49942..50904	+	963	WP_119798014.1	thioredoxin fold domain-containing protein	-
CCS41_RS15365	50907..53057	+	2151	WP_119798015.1	conjugal transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67447	67447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-97)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11477.23 Da
• Isoelectric Point: 6.4684

>T76925 WP_119798012.1 NZ_CP021662:c48312-48007 [Candidatus Fukatsuia symbiotica]
VPQIKIIITEGAAQGLERCRHFLAEKNPLASIRAGQSIEKQFVLLEKEPEMGRPLDDQPTLRELMIPFGKSGYVALYRFE
AETDSVYVLAFRHQKEAGFKD

Nucleotide

• Length: 306 bp

>T76925 NZ_CP021662:c48312-48007 [Candidatus Fukatsuia symbiotica]
GTGCCACAAATAAAAATAATTATCACCGAAGGAGCAGCCCAAGGTTTAGAGCGTTGTCGGCATTTTTTGGCAGAAAAAAA
TCCACTGGCTTCAATAAGAGCCGGTCAATCTATCGAAAAGCAATTTGTACTGCTGGAGAAGGAACCCGAAATGGGTCGAC
CGCTTGATGACCAACCCACGTTGCGAGAATTGATGATCCCTTTTGGCAAATCTGGCTATGTGGCGCTCTATCGTTTTGAG
GCAGAAACCGATTCGGTATACGTGCTAGCTTTTCGTCATCAGAAAGAAGCTGGATTTAAAGACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.15 Da
• Isoelectric Point: 9.0274

>AT76925 WP_119798013.1 NZ_CP021662:c48572-48300 [Candidatus Fukatsuia symbiotica]
MATSIKLDDHLKTRIHHLAERQRRSAHWIMREAIRDYVDREEKRENFKQEALGSWAIYQKTGRHLSGDEVRGWLQTWGTD
KETEIPPCHK

Nucleotide

• Length: 273 bp

>AT76925 NZ_CP021662:c48572-48300 [Candidatus Fukatsuia symbiotica]
ATGGCGACCTCGATCAAACTTGATGACCATTTGAAAACTCGCATTCATCACCTTGCCGAGAGGCAACGCCGTTCGGCGCA
TTGGATCATGCGTGAAGCGATACGTGATTATGTTGATCGCGAGGAAAAACGTGAAAATTTCAAGCAAGAAGCGCTGGGAT
CCTGGGCGATTTATCAGAAAACAGGGCGGCATCTTAGTGGTGATGAAGTGCGTGGCTGGTTGCAGACCTGGGGTACCGAT
AAAGAAACAGAGATACCCCCGTGCCACAAATAA