Detailed information of TA system TA076924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 39042..39691
• Replicon: plasmid p5D_Fsymbiotica-3
• Accession: NZ_CP021662
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCS41_RS15305
• Protein ID: WP_119798008.1
• Coordinates: 39042..39461 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCS41_RS15310
• Protein ID: WP_119798009.1
• Coordinates: 39461..39691 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS15265	34132..34356	-	225	WP_119798024.1	TraY domain-containing protein	-
CCS41_RS15275	34509..35404	+	896	Protein_36	conjugal transfer protein TraN	-
CCS41_RS15280	35408..36619	+	1212	WP_119798004.1	conjugal transfer protein TraO	-
CCS41_RS15285	36616..37371	+	756	WP_119798005.1	conjugal transfer protein TraP	-
CCS41_RS15850	37382..37576	+	195	WP_162860139.1	hypothetical protein	-
CCS41_RS15290	37561..37923	+	363	WP_162860140.1	conjugal transfer protein TraQ	-
CCS41_RS15295	37991..38386	+	396	WP_119798006.1	TraR conjugal transfer protein	-
CCS41_RS15300	38435..39013	+	579	WP_119798007.1	TraT conjugal transfer protein	-
CCS41_RS15305	39042..39461	-	420	WP_119798008.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CCS41_RS15310	39461..39691	-	231	WP_119798009.1	antitoxin	Antitoxin
CCS41_RS15315	39819..42889	+	3071	Protein_45	ATP-binding protein	-
CCS41_RS15320	42899..44092	+	1194	WP_162860111.1	conjugal transfer protein TraW	-
CCS41_RS15325	44085..44645	+	561	WP_119797797.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67447	67447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15669.98 Da
• Isoelectric Point: 7.3218

>T76924 WP_119798008.1 NZ_CP021662:c39461-39042 [Candidatus Fukatsuia symbiotica]
MLKYMLDTNICIFTIKNKPQEVRATFDRHHGQLCISTVTLMELIYGAEKSHSAEKNLRVIEGFVARLEVLNYTQKAAADS
GQLRAELANNGTPIGPYDQMIAGHARSQGLILVTNNLREFERVPGLRVEDWVINNTKID

Nucleotide

• Length: 420 bp

>T76924 NZ_CP021662:c39461-39042 [Candidatus Fukatsuia symbiotica]
ATGCTGAAATATATGCTCGATACCAATATTTGCATCTTTACTATCAAGAATAAGCCACAGGAAGTACGGGCCACATTCGA
TCGCCATCACGGCCAACTCTGTATCAGCACAGTGACGTTAATGGAGCTAATTTATGGTGCTGAAAAATCGCATTCTGCCG
AGAAAAATTTACGGGTTATTGAAGGATTCGTCGCGCGCCTTGAAGTACTCAATTACACGCAGAAAGCGGCTGCCGATAGT
GGTCAGCTTAGGGCTGAACTGGCTAACAATGGCACCCCGATTGGTCCCTACGATCAAATGATCGCGGGCCATGCTCGCTC
ACAAGGATTAATACTGGTGACAAACAATTTACGTGAATTCGAGCGTGTACCTGGCTTACGTGTTGAGGATTGGGTGATCA
ATAACACTAAAATCGATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8696.73 Da
• Isoelectric Point: 4.7719

>AT76924 WP_119798009.1 NZ_CP021662:c39691-39461 [Candidatus Fukatsuia symbiotica]
MEQTSIFKSNRSQAVRLPKAVALPEDVKRVDIVAIGRTRIITPAGESWDSWFDGESVSADFMVNREQPRDQERETF

Nucleotide

• Length: 231 bp

>AT76924 NZ_CP021662:c39691-39461 [Candidatus Fukatsuia symbiotica]
ATGGAACAAACCTCAATATTTAAAAGCAATCGCAGCCAGGCTGTTCGCCTACCTAAAGCAGTAGCGCTACCAGAAGACGT
CAAACGGGTTGACATTGTGGCTATAGGCCGTACCCGTATCATCACACCGGCAGGAGAGTCCTGGGATAGCTGGTTTGATG
GGGAAAGTGTATCTGCTGACTTTATGGTGAATCGTGAGCAACCCAGAGATCAAGAACGAGAAACATTTTGA