Detailed information of TA system TA076923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 33876..34356
• Replicon: plasmid p5D_Fsymbiotica-3
• Accession: NZ_CP021662
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: CCS41_RS15260
• Protein ID: WP_119798001.1
• Coordinates: 33876..34148 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: CCS41_RS15265
• Protein ID: WP_119798024.1
• Coordinates: 34132..34356 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS15230	29738..30760	-	1023	WP_119797794.1	IS630 family transposase	-
CCS41_RS16200	30822..30950	+	129	Protein_28	GNAT family N-acetyltransferase	-
CCS41_RS15235	30947..31351	-	405	Protein_29	transposase	-
CCS41_RS15240	31498..31770	-	273	WP_162860138.1	IS630 transposase-related protein	-
CCS41_RS15245	31919..32457	-	539	Protein_31	recombinase family protein	-
CCS41_RS15250	32627..33450	+	824	Protein_32	IS5 family transposase	-
CCS41_RS15255	33469..33816	+	348	Protein_33	DotI/IcmL/TraM family protein	-
CCS41_RS15260	33876..34148	-	273	WP_119798001.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCS41_RS15265	34132..34356	-	225	WP_119798024.1	TraY domain-containing protein	Antitoxin
CCS41_RS15275	34509..35404	+	896	Protein_36	conjugal transfer protein TraN	-
CCS41_RS15280	35408..36619	+	1212	WP_119798004.1	conjugal transfer protein TraO	-
CCS41_RS15285	36616..37371	+	756	WP_119798005.1	conjugal transfer protein TraP	-
CCS41_RS15850	37382..37576	+	195	WP_162860139.1	hypothetical protein	-
CCS41_RS15290	37561..37923	+	363	WP_162860140.1	conjugal transfer protein TraQ	-
CCS41_RS15295	37991..38386	+	396	WP_119798006.1	TraR conjugal transfer protein	-
CCS41_RS15300	38435..39013	+	579	WP_119798007.1	TraT conjugal transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67447	67447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-47)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10660.60 Da
• Isoelectric Point: 10.9153

>T76923 WP_119798001.1 NZ_CP021662:c34148-33876 [Candidatus Fukatsuia symbiotica]
MTWKVELSTLARKNLGDLDKQIAKRILRFLHDRIAKLENPRDVGDALKGIKLGVFWKYRVGDYRIIADIQDRELIILVVR
IGNRREVYKR

Nucleotide

• Length: 273 bp

>T76923 NZ_CP021662:c34148-33876 [Candidatus Fukatsuia symbiotica]
ATGACTTGGAAAGTTGAGCTGTCTACACTGGCTCGGAAAAATTTGGGCGATTTAGATAAACAAATCGCTAAACGTATTTT
ACGATTTTTACATGATCGTATTGCCAAGTTAGAAAATCCACGAGACGTCGGCGACGCCTTAAAGGGCATTAAATTAGGTG
TATTTTGGAAATACCGTGTGGGTGACTATCGCATCATCGCCGATATTCAAGATCGCGAATTGATCATTCTGGTTGTGCGT
ATTGGCAACCGCCGCGAAGTTTACAAACGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8614.79 Da
• Isoelectric Point: 4.4389

>AT76923 WP_119798024.1 NZ_CP021662:c34356-34132 [Candidatus Fukatsuia symbiotica]
MLAIRLPQEIENRLKKLADVTGRSKTFYAREAILEHLDDLEDIYLAEHELEEVRAGRSVPVSLEEVMKQYDLES

Nucleotide

• Length: 225 bp

>AT76923 NZ_CP021662:c34356-34132 [Candidatus Fukatsuia symbiotica]
ATGCTTGCCATCAGACTCCCTCAAGAAATTGAAAACCGGTTGAAAAAATTGGCCGATGTGACGGGGCGTAGCAAAACTTT
TTATGCGCGTGAAGCCATCCTAGAGCACCTCGATGATTTGGAAGACATTTACCTTGCTGAGCATGAACTTGAAGAAGTGC
GCGCTGGCCGTAGTGTCCCTGTGTCTCTGGAAGAAGTGATGAAACAATATGACTTGGAAAGTTGA