Detailed information of TA system TA076921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-vagC/HigB-VagC
• Location: 44944..45566
• Replicon: plasmid p5D_Fsymbiotica-2
• Accession: NZ_CP021661
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CCS41_RS14805
• Protein ID: WP_119797938.1
• Coordinates: 44944..45255 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CCS41_RS14810
• Protein ID: WP_119797939.1
• Coordinates: 45333..45566 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS14760	39981..40784	-	804	WP_119797931.1	TrbG/VirB9 family P-type conjugative transfer protein	-
CCS41_RS14765	40789..41589	-	801	WP_119797932.1	type IV secretion system protein	-
CCS41_RS14770	41802..42752	-	951	WP_119797933.1	type IV secretion system protein	-
CCS41_RS14775	42776..43117	-	342	WP_039908292.1	hypothetical protein	-
CCS41_RS14780	43158..43535	-	378	WP_119797934.1	helix-turn-helix domain-containing protein	-
CCS41_RS14785	43529..43924	-	396	WP_119797935.1	type II toxin-antitoxin system VapC family toxin	-
CCS41_RS14790	43921..44157	-	237	WP_006705272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CCS41_RS14795	44239..44496	-	258	WP_119797936.1	hypothetical protein	-
CCS41_RS14800	44537..44947	-	411	WP_119797937.1	helix-turn-helix domain-containing protein	-
CCS41_RS14805	44944..45255	-	312	WP_119797938.1	type II toxin-antitoxin system HigB family toxin	Toxin
CCS41_RS14810	45333..45566	-	234	WP_119797939.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CCS41_RS14815	45631..46323	-	693	WP_119797940.1	type IV secretion system protein	-
CCS41_RS14820	46320..48716	-	2397	WP_119797941.1	VirB4 family type IV secretion system protein	-
CCS41_RS14825	48673..49043	-	371	Protein_66	VirB3 family type IV secretion system protein	-
CCS41_RS14830	49040..49384	-	345	WP_119797942.1	hypothetical protein	-
CCS41_RS14835	49338..50030	-	693	WP_205411523.1	transglycosylase SLT domain-containing protein	-
CCS41_RS14840	50074..50298	-	225	WP_119797943.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91823	91823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• SpoVT-AbrB domain (8-45)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12666.63 Da
• Isoelectric Point: 10.1450

>T76921 WP_119797938.1 NZ_CP021661:c45255-44944 [Candidatus Fukatsuia symbiotica]
MHIISKEPFEAAHRKYGRDAYAIVSTYKYLKDNNFKNPEELKNIFPSLDKFKYRDKWWVIDIGGNHLRLIVYINFINKRV
YIKHIVTHSEYDKLNKYYREHKK

Nucleotide

• Length: 312 bp

>T76921 NZ_CP021661:c45255-44944 [Candidatus Fukatsuia symbiotica]
ATGCACATAATATCAAAAGAACCCTTTGAAGCTGCACATAGAAAATACGGGAGAGACGCCTATGCGATAGTCTCAACGTA
TAAATATCTGAAAGACAATAATTTTAAAAATCCTGAGGAACTCAAAAACATCTTTCCCAGTTTGGATAAATTCAAATATA
GAGACAAATGGTGGGTCATTGATATAGGTGGAAATCATTTAAGATTAATCGTTTATATTAATTTTATTAATAAACGTGTT
TACATCAAACATATCGTGACACATTCTGAATATGACAAATTGAATAAATATTATCGGGAGCATAAAAAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8881.16 Da
• Isoelectric Point: 4.8096

>AT76921 WP_119797939.1 NZ_CP021661:c45566-45333 [Candidatus Fukatsuia symbiotica]
MKRTVAIFKNGRNRAIRLPVEMDFAGVNELLIEREGDTLMLKPIRPTWTTFTDNSEKADADFLLDRETVLEEGHFSL

Nucleotide

• Length: 234 bp

>AT76921 NZ_CP021661:c45566-45333 [Candidatus Fukatsuia symbiotica]
ATGAAGAGAACGGTCGCCATTTTTAAAAATGGGAGAAACCGCGCCATCAGGTTGCCTGTAGAGATGGATTTTGCTGGCGT
GAATGAACTGCTGATTGAACGGGAAGGCGATACGCTGATGTTAAAACCCATTCGCCCCACTTGGACAACCTTCACAGACA
ACAGCGAAAAGGCGGATGCCGATTTTCTGCTGGATCGGGAAACCGTTCTTGAAGAAGGGCATTTTTCCCTGTAA