Detailed information of TA system TA076918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 21079..21775
• Replicon: plasmid p5D_Fsymbiotica-2
• Accession: NZ_CP021661
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: CCS41_RS14620
• Protein ID: WP_119797911.1
• Coordinates: 21079..21459 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CCS41_RS14625
• Protein ID: WP_119797912.1
• Coordinates: 21440..21775 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS14590	16136..16843	+	708	WP_119797905.1	plasmid stability protein StbB	-
CCS41_RS14595	16853..17449	+	597	WP_119797906.1	hypothetical protein	-
CCS41_RS14600	17541..17846	-	306	WP_119797907.1	hypothetical protein	-
CCS41_RS14605	17892..18545	-	654	WP_119797908.1	ParA family protein	-
CCS41_RS14610	18675..18920	-	246	WP_119797909.1	hypothetical protein	-
CCS41_RS14615	18886..21021	-	2136	WP_119797910.1	type IA DNA topoisomerase	-
CCS41_RS14620	21079..21459	+	381	WP_119797911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCS41_RS14625	21440..21775	+	336	WP_119797912.1	helix-turn-helix domain-containing protein	Antitoxin
CCS41_RS14630	21827..22333	-	507	WP_119797913.1	lytic transglycosylase domain-containing protein	-
CCS41_RS14635	22349..22969	-	621	WP_205411528.1	prepilin peptidase	-
CCS41_RS14640	22972..23403	-	432	WP_119797915.1	type IV pilus biogenesis protein PilM	-
CCS41_RS14645	23443..24714	-	1272	WP_119797916.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
CCS41_RS14650	24730..25689	-	960	WP_119797917.1	Flp pilus assembly complex ATPase component TadA	-
CCS41_RS14655	25699..26211	-	513	WP_119797976.1	pilus assembly protein PilS	-
CCS41_RS14660	26250..26759	-	510	WP_119797918.1	pilus assembly protein PilS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91823	91823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14068.36 Da
• Isoelectric Point: 10.3205

>T76918 WP_119797911.1 NZ_CP021661:21079-21459 [Candidatus Fukatsuia symbiotica]
MITQEKQLIWIGSSKKDLMALPTNVRKFFGHALDFAQRGDQHDTAKVMKGFGGAGVLEIIEKDLGGTYRAVYTVKFEEAV
FVLHCFQKKSKSGIATPKEDMNIIHARLKVAAAFAKRLLNNDKTNN

Nucleotide

• Length: 381 bp

>T76918 NZ_CP021661:21079-21459 [Candidatus Fukatsuia symbiotica]
ATGATTACACAAGAAAAACAGCTCATCTGGATTGGAAGCAGCAAAAAAGACTTGATGGCGCTCCCTACCAACGTGCGAAA
ATTCTTCGGTCATGCCCTGGATTTCGCCCAACGCGGCGATCAGCACGACACGGCGAAAGTAATGAAAGGGTTCGGTGGGG
CAGGTGTTTTAGAAATCATTGAAAAGGATCTCGGTGGCACTTATCGCGCTGTCTACACTGTGAAGTTTGAAGAAGCGGTG
TTCGTTCTGCATTGCTTCCAAAAAAAGAGCAAAAGTGGGATTGCTACGCCAAAAGAGGACATGAATATCATTCACGCCCG
CCTTAAGGTAGCAGCAGCGTTTGCAAAGAGGTTACTAAATAATGACAAAACAAATAATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12031.20 Da
• Isoelectric Point: 9.7405

>AT76918 WP_119797912.1 NZ_CP021661:21440-21775 [Candidatus Fukatsuia symbiotica]
MTKQIIEGIEVEICSSNIFADMGLPNASKLKIKSGLVVEITKAIRKLDLTQQEAASRMGITQSKVSGMLRGDFSNLSERK
LMNCLNRLGYDIEIKVKPAAASLGHVILAVA

Nucleotide

• Length: 336 bp

>AT76918 NZ_CP021661:21440-21775 [Candidatus Fukatsuia symbiotica]
ATGACAAAACAAATAATTGAAGGCATTGAGGTCGAAATCTGTTCCAGCAACATTTTTGCCGATATGGGTTTGCCAAATGC
GAGCAAGTTAAAGATCAAGTCTGGTCTGGTAGTTGAAATCACCAAAGCTATACGTAAGCTGGATCTTACTCAACAGGAGG
CAGCGAGCCGTATGGGTATTACGCAGTCAAAAGTTTCCGGCATGTTGAGGGGAGATTTCTCCAACCTCTCTGAGCGCAAG
CTAATGAACTGCTTGAATCGGCTTGGTTACGATATTGAAATAAAAGTGAAACCGGCGGCAGCATCTCTTGGGCATGTAAT
CCTTGCTGTCGCTTAA