Detailed information of TA system TA076917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yhaV-higA/YhaV-MqsA
• Location: 143389..144367
• Replicon: plasmid p5D_Fsymbiotica-1
• Accession: NZ_CP021660
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CCS41_RS14475
• Protein ID: WP_119797885.1
• Coordinates: 143849..144367 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCS41_RS14465
• Protein ID: WP_119797884.1
• Coordinates: 143389..143703 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS14425	139060..139809	+	750	Protein_132	transposase	-
CCS41_RS14430	140143..140772	+	630	WP_119797879.1	AAA family ATPase	-
CCS41_RS14435	140769..141002	+	234	WP_119797880.1	hypothetical protein	-
CCS41_RS14440	141102..141463	+	362	Protein_135	IS630 family transposase	-
CCS41_RS14445	141565..141876	+	312	WP_119797893.1	type II toxin-antitoxin system HigB family toxin	-
CCS41_RS14450	141873..142337	+	465	WP_119797881.1	helix-turn-helix domain-containing protein	-
CCS41_RS14455	142442..142735	+	294	WP_119797882.1	DUF1778 domain-containing protein	-
CCS41_RS14460	142732..143232	+	501	WP_119797883.1	GNAT family N-acetyltransferase	-
CCS41_RS14465	143389..143703	-	315	WP_119797884.1	helix-turn-helix domain-containing protein	Antitoxin
CCS41_RS14475	143849..144367	-	519	WP_119797885.1	type II toxin-antitoxin system YhaV family toxin	Toxin
CCS41_RS14480	144376..144741	-	366	WP_119797886.1	type II toxin-antitoxin system PrlF family antitoxin	-
CCS41_RS14485	144933..145914	+	982	Protein_143	transposase	-
CCS41_RS14490	146034..146303	-	270	WP_119797887.1	hypothetical protein	-
CCS41_RS14495	146303..146938	-	636	WP_119797888.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..148316	148316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (14-149)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-88)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 20244.46 Da
• Isoelectric Point: 9.9230

>T76917 WP_119797885.1 NZ_CP021660:c144367-143849 [Candidatus Fukatsuia symbiotica]
MNERPYKINGWYVIVHPLFTRQIEELTNEVEKLKASHPDTYQKKKVTLRLAAIKHLILNDITIDPSNTKFRLGGTLGDDY
KHWFRAKFYQQYRLFFRYESASKIIILVWVNDDNTLRIYKAKNDAYKVFKGMLDDGNPPDDWGKLLSVCKTAQMNDNLEK
HSAMKTKIIKVC

Nucleotide

• Length: 519 bp

>T76917 NZ_CP021660:c144367-143849 [Candidatus Fukatsuia symbiotica]
ATGAACGAGCGACCCTATAAAATAAATGGATGGTATGTCATAGTTCACCCTCTGTTTACAAGGCAAATTGAAGAACTCAC
TAACGAGGTTGAGAAACTTAAAGCCAGCCATCCTGACACCTATCAAAAGAAAAAAGTGACTCTTCGTCTGGCAGCTATAA
AACATCTGATACTAAACGATATAACAATAGATCCATCCAACACTAAGTTTCGTTTAGGTGGAACGTTAGGTGATGATTAT
AAACACTGGTTCAGAGCAAAGTTCTATCAGCAATACCGCCTATTCTTCCGCTATGAATCAGCCTCTAAAATCATCATCCT
TGTCTGGGTGAATGACGATAATACTCTAAGGATCTATAAGGCAAAAAATGATGCATATAAAGTATTCAAAGGCATGCTAG
ATGATGGAAACCCGCCAGATGATTGGGGTAAGCTGCTGTCTGTATGTAAAACAGCGCAAATGAATGATAATCTGGAAAAA
CATTCAGCAATGAAAACAAAAATTATTAAAGTCTGTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11685.54 Da
• Isoelectric Point: 9.6573

>AT76917 WP_119797884.1 NZ_CP021660:c143703-143389 [Candidatus Fukatsuia symbiotica]
MNERNIFDELMQGMEAWGNMNAGKETLKIHRLSSNKGITLCPSELKALREKLNLSQAVFAQYLHTGVTTYQNWEQGRAKP
NQQVVLLIKMVEKNPATLSALAAL

Nucleotide

• Length: 315 bp

>AT76917 NZ_CP021660:c143703-143389 [Candidatus Fukatsuia symbiotica]
ATGAACGAACGTAATATCTTTGATGAACTAATGCAAGGAATGGAAGCCTGGGGTAATATGAACGCTGGGAAAGAAACGCT
AAAAATTCATCGTCTCTCCTCGAACAAAGGCATCACCTTATGCCCTTCAGAACTAAAAGCGTTAAGAGAAAAACTTAACC
TTTCTCAAGCCGTTTTTGCCCAGTATCTGCATACTGGCGTGACAACTTATCAAAACTGGGAACAAGGCAGGGCGAAGCCG
AATCAACAAGTCGTGTTACTCATTAAAATGGTAGAAAAAAATCCGGCTACACTTTCTGCATTAGCCGCGCTGTGA