Detailed information of TA system TA076914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 122048..122687
• Replicon: plasmid p5D_Fsymbiotica-1
• Accession: NZ_CP021660
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CCS41_RS14325
• Protein ID: WP_119797865.1
• Coordinates: 122048..122230 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CCS41_RS14330
• Protein ID: WP_119797866.1
• Coordinates: 122259..122687 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS15795	117297..117449	-	153	WP_162860119.1	hypothetical protein	-
CCS41_RS14300	117446..118156	-	711	WP_119797860.1	PAS domain-containing protein	-
CCS41_RS14305	118832..119563	+	732	WP_119797861.1	LuxR family transcriptional regulator	-
CCS41_RS14310	119955..120587	+	633	WP_119797862.1	acyl-homoserine-lactone synthase	-
CCS41_RS14320	121098..121832	+	735	WP_119797864.1	replication initiation protein	-
CCS41_RS14325	122048..122230	+	183	WP_119797865.1	type II toxin-antitoxin system HicA family toxin	Toxin
CCS41_RS14330	122259..122687	+	429	WP_119797866.1	hypothetical protein	Antitoxin
CCS41_RS14335	122881..123150	-	270	WP_119797867.1	transcriptional regulator	-
CCS41_RS14340	123994..124296	+	303	WP_119797868.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CCS41_RS14345	124387..124818	+	432	WP_119797869.1	IS200/IS605 family transposase	-
CCS41_RS14350	124857..125045	-	189	Protein_118	hypothetical protein	-
CCS41_RS14355	125231..125539	-	309	WP_119797870.1	hypothetical protein	-
CCS41_RS14360	125606..126253	-	648	WP_119797871.1	ParA family protein	-
CCS41_RS14365	126501..127016	-	516	WP_119797872.1	GNAT family N-acetyltransferase	-
CCS41_RS14370	127000..127293	-	294	WP_119797873.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..148316	148316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6856.03 Da
• Isoelectric Point: 10.8431

>T76914 WP_119797865.1 NZ_CP021660:122048-122230 [Candidatus Fukatsuia symbiotica]
MKYSEFRRWLISQGVLIEKARGGGSHRKVSVNGKSYVFPDHGSKEMPEPLRKKIIKDLGL

Nucleotide

• Length: 183 bp

>T76914 NZ_CP021660:122048-122230 [Candidatus Fukatsuia symbiotica]
ATGAAATATAGTGAATTCCGTCGCTGGTTGATTTCTCAGGGTGTTTTGATTGAAAAGGCCAGGGGAGGAGGGAGTCACCG
TAAGGTGTCGGTAAATGGCAAATCATATGTTTTTCCAGACCATGGTTCTAAAGAAATGCCTGAACCGTTGAGAAAGAAAA
TAATCAAGGATTTGGGTCTTTAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15741.99 Da
• Isoelectric Point: 4.5912

>AT76914 WP_119797866.1 NZ_CP021660:122259-122687 [Candidatus Fukatsuia symbiotica]
MFNYPIRLEYDKETAVYVASCRDLPLFNSVGDSVDEALLESVDGLITAISIEIDERRPISNASAVEDGEYVLSLPILVTM
KAALHNAMIETGTRKADLARRLGQKGPQIDRLLDVEHSSKVETVELALHQLDRKLDIAVHVV

Nucleotide

• Length: 429 bp

>AT76914 NZ_CP021660:122259-122687 [Candidatus Fukatsuia symbiotica]
ATGTTTAACTATCCAATCAGGCTAGAATATGATAAGGAAACAGCGGTTTATGTGGCTAGCTGCCGTGATCTTCCCTTGTT
TAATTCGGTCGGTGACTCCGTTGATGAAGCTCTTCTCGAATCTGTAGATGGGTTGATTACAGCTATTTCTATTGAAATTG
ACGAACGTCGGCCTATATCCAATGCATCCGCCGTGGAAGATGGAGAATACGTGTTAAGTTTACCCATTCTGGTCACAATG
AAAGCGGCTTTACATAATGCGATGATCGAGACGGGGACCCGCAAGGCAGATCTTGCTCGTCGACTAGGGCAAAAGGGACC
GCAGATTGACCGACTTTTGGATGTAGAGCACTCATCAAAAGTTGAAACGGTAGAGTTGGCGCTGCACCAGCTAGATAGAA
AGTTAGATATTGCCGTTCACGTCGTATAA