Detailed information of TA system TA076913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 35396..36042
• Replicon: plasmid p5D_Fsymbiotica-1
• Accession: NZ_CP021660
• Organism: Candidatus Fukatsuia symbiotica strain 5D

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCS41_RS13990
• Protein ID: WP_119797891.1
• Coordinates: 35647..36042 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCS41_RS13985
• Protein ID: WP_119797809.1
• Coordinates: 35396..35638 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS41_RS13960	31056..31740	-	685	Protein_40	DotI/IcmL/TraM family protein	-
CCS41_RS13965	31765..32199	-	435	WP_119797805.1	conjugal transfer protein TraL	-
CCS41_RS13970	32220..33143	-	924	WP_119797806.1	hypothetical protein	-
CCS41_RS13975	33200..35011	-	1812	WP_119797807.1	DNA primase	-
CCS41_RS13980	35024..35284	-	261	WP_119797808.1	IcmT/TraK family protein	-
CCS41_RS13985	35396..35638	+	243	WP_119797809.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CCS41_RS13990	35647..36042	+	396	WP_119797891.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCS41_RS15785	35995..36567	-	573	WP_119963870.1	hypothetical protein	-
CCS41_RS13995	36598..36801	+	204	WP_119797810.1	type II toxin-antitoxin system YafQ family toxin	-
CCS41_RS14000	36816..37982	-	1167	WP_119797811.1	plasmid transfer ATPase TraJ	-
CCS41_RS14005	37984..38772	-	789	WP_119797812.1	type IV secretion system DotC family protein	-
CCS41_RS14010	38769..39218	-	450	WP_119797813.1	DotD/TraH family lipoprotein	-
CCS41_RS14015	39258..39449	-	192	WP_119797814.1	Rop family plasmid primer RNA-binding protein	-
CCS41_RS14020	39560..40921	-	1362	WP_119797815.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..148316	148316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-119)

Antitoxin

• SpoVT-AbrB domain (8-53)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14036.49 Da
• Isoelectric Point: 6.7155

>T76913 WP_119797891.1 NZ_CP021660:35647-36042 [Candidatus Fukatsuia symbiotica]
MLDTNMASYVIKGNPPEARQRLAVLPMESIVISVVTQAELLYGLARKGHPVTFAGLIREFFLRVKILPWDGEAATVYGDL
RASCAAAGITLSALDMLIAAHAIAAKATLVTHDKAFSLVPDGMLTVADWIT

Nucleotide

• Length: 396 bp

>T76913 NZ_CP021660:35647-36042 [Candidatus Fukatsuia symbiotica]
ATGCTAGATACGAACATGGCAAGCTATGTTATCAAAGGTAATCCACCGGAAGCGCGCCAGCGATTGGCTGTGCTACCGAT
GGAAAGCATCGTCATCTCGGTCGTTACACAAGCTGAATTGCTTTACGGACTCGCCCGTAAAGGCCATCCAGTCACCTTTG
CGGGCCTCATACGCGAATTTTTTCTTCGTGTTAAAATACTGCCTTGGGATGGCGAAGCGGCAACCGTTTACGGGGACTTG
CGTGCTTCTTGCGCCGCTGCCGGTATCACCCTTAGCGCATTAGATATGCTGATTGCCGCGCATGCGATAGCAGCTAAAGC
GACTTTGGTAACCCACGACAAGGCTTTTTCACTCGTCCCTGATGGGATGCTTACTGTTGCAGACTGGATCACTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9168.26 Da
• Isoelectric Point: 4.4230

>AT76913 WP_119797809.1 NZ_CP021660:35396-35638 [Candidatus Fukatsuia symbiotica]
MSTTAKLFTTGRSQAVRLPMEFRFKGSEVFIRRNQDTGEVVLSSKPASWQDFFALADSTDIPEDFMVDRENLSAEERDLF

Nucleotide

• Length: 243 bp

>AT76913 NZ_CP021660:35396-35638 [Candidatus Fukatsuia symbiotica]
ATGTCAACTACAGCCAAACTGTTCACTACCGGTCGCAGTCAAGCCGTACGGCTCCCGATGGAGTTCCGATTCAAGGGATC
AGAAGTTTTTATTCGTCGTAACCAGGATACGGGCGAGGTTGTTTTATCGTCTAAGCCAGCATCCTGGCAAGATTTTTTCG
CGCTAGCCGATAGCACTGATATTCCGGAAGATTTCATGGTTGATCGTGAAAATCTATCCGCTGAAGAAAGGGATCTATTT
TGA