Detailed information of TA system TA076902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 9792..10432
• Replicon: plasmid pO23AP4
• Accession: NZ_CP021658
• Organism: Aeromonas salmonicida strain O23A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: O23A_RS22645
• Protein ID: WP_087757564.1
• Coordinates: 10256..10432 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: O23A_RS22640
• Protein ID: WP_087757563.1
• Coordinates: 9792..10205 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O23A_RS22605	5048..5431	-	384	WP_087757557.1	DNA repair protein	-
O23A_RS22610	5472..5918	-	447	WP_087757558.1	DNA repair protein RadC	-
O23A_RS22615	6008..6370	-	363	WP_087757559.1	hypothetical protein	-
O23A_RS22620	7289..7696	-	408	WP_087757560.1	hypothetical protein	-
O23A_RS22625	7711..7908	-	198	WP_087757561.1	hypothetical protein	-
O23A_RS23350	8011..8160	-	150	WP_157666857.1	hypothetical protein	-
O23A_RS22630	8171..8800	-	630	WP_087757562.1	hypothetical protein	-
O23A_RS23355	8899..9072	-	174	WP_157666858.1	hypothetical protein	-
O23A_RS23360	9556..9711	+	156	WP_157666859.1	hypothetical protein	-
O23A_RS22640	9792..10205	-	414	WP_087757563.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
O23A_RS22645	10256..10432	-	177	WP_087757564.1	type II toxin-antitoxin system HicA family toxin	Toxin
O23A_RS22650	11183..11470	+	288	WP_087757565.1	hypothetical protein	-
O23A_RS22655	11503..11718	+	216	WP_087757615.1	hypothetical protein	-
O23A_RS22665	12077..12463	-	387	WP_087757567.1	hypothetical protein	-
O23A_RS22670	12561..12890	-	330	WP_087757568.1	hypothetical protein	-
O23A_RS22675	13092..13346	-	255	WP_087757569.1	hypothetical protein	-
O23A_RS22680	13764..14186	+	423	WP_087757616.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60010	60010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6791.89 Da
• Isoelectric Point: 11.1658

>T76902 WP_087757564.1 NZ_CP021658:c10432-10256 [Aeromonas salmonicida]
VKQSEFRRWLESQGVDIRQGTNHLKLYYKGRQSTMPRHPSKELGEGLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T76902 NZ_CP021658:c10432-10256 [Aeromonas salmonicida]
GTGAAGCAAAGCGAGTTTCGGCGATGGCTGGAGTCGCAAGGAGTAGACATCAGGCAAGGCACCAACCACCTGAAACTCTA
CTACAAAGGGCGCCAGTCAACGATGCCGAGGCACCCCAGCAAGGAGCTGGGGGAGGGTTTAAGAAAAGCTATCCTCAAGC
AACTCGGCATCAAATAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15117.20 Da
• Isoelectric Point: 4.9192

>AT76902 WP_087757563.1 NZ_CP021658:c10205-9792 [Aeromonas salmonicida]
MHYPVKIERDGDTWMASFPDVPEALTGGDTREEALAEALDALITAFEFYFEGNRPVPQPSTPATGQEVVTVPPSLWAKVL
LLNAMCESSVNQAELGRRLGMPRQNVQRLTDLHHATKIDQLANAVSALGHRLELVIR

Nucleotide

• Length: 414 bp

>AT76902 NZ_CP021658:c10205-9792 [Aeromonas salmonicida]
ATGCACTACCCTGTAAAAATTGAACGTGATGGTGACACTTGGATGGCGAGCTTCCCGGATGTACCGGAAGCGCTGACCGG
TGGAGATACCCGTGAGGAGGCGCTGGCCGAGGCGCTGGATGCGCTGATCACCGCCTTCGAGTTCTACTTTGAAGGTAACC
GTCCGGTGCCGCAGCCCTCAACGCCTGCCACTGGTCAGGAGGTCGTGACTGTGCCCCCCAGCTTGTGGGCCAAGGTGCTG
CTGCTCAACGCCATGTGCGAGTCGAGTGTCAACCAAGCTGAGCTGGGTCGCCGTCTGGGGATGCCACGCCAGAACGTGCA
GCGCCTGACCGACCTGCACCATGCCACCAAGATCGACCAGTTGGCCAACGCCGTGTCGGCACTTGGCCACCGGCTCGAAC
TGGTGATCCGCTAA