Detailed information of TA system TA076901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 1877566..1878227
• Replicon: plasmid unnamed
• Accession: NZ_CP021653
• Organism: Ralstonia solanacearum strain RS 488

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A399W071
• Locus tag: CCY86_RS23405
• Protein ID: WP_003278076.1
• Coordinates: 1877814..1878227 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCY86_RS23400
• Protein ID: WP_003278075.1
• Coordinates: 1877566..1877817 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCY86_RS23390	1872990..1873385	-	396	WP_039559478.1	hypothetical protein	-
CCY86_RS23395	1873391..1877491	-	4101	WP_039559476.1	phage tail tape measure protein	-
CCY86_RS23400	1877566..1877817	+	252	WP_003278075.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CCY86_RS23405	1877814..1878227	+	414	WP_003278076.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCY86_RS23415	1878686..1879177	-	492	Protein_1461	IS5 family transposase	-
CCY86_RS23425	1879762..1880567	+	806	WP_086005266.1	IS5 family transposase	-
CCY86_RS23430	1881144..1882018	-	875	Protein_1463	IS5 family transposase	-
CCY86_RS23435	1882466..1882756	+	291	WP_039559430.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999547	1999547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15756.12 Da
• Isoelectric Point: 6.2337

>T76901 WP_003278076.1 NZ_CP021653:1877814-1878227 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKQPDARVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLETELPNYFLGRLLDID
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Nucleotide

• Length: 414 bp

>T76901 NZ_CP021653:1877814-1878227 [Ralstonia solanacearum]
TTGAGTTACCTGATCGACACCAATGTGCTGTCCGAACTACGCCGCAAGCAGCCCGATGCGCGCGTGCTCGCCTGGATGCA
AGACCGGCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAAATCCGCAAAGGGATCGAGCGGCTGGACGACG
CAGCACGCCGCCAGCACCTGATCGACTGGCTCGAAACGGAACTGCCGAACTACTTCCTCGGCCGCCTGCTCGACATCGAT
GCGCATACCGCCGACCGCTGGGGCCGACTGATGTCGAGCGCCAACCGGCCGCTGCCTGCGATCGATGGCCTGCTCGCCGC
GACCGCCCTGCAGCACGACCTCATACTGGTCACGCGTAACACGAAGGATTTCGTCGGACTCGATGTCCCGCTCATCAACC
CTTGGGAAACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9242.46 Da
• Isoelectric Point: 5.0774

>AT76901 WP_003278075.1 NZ_CP021653:1877566-1877817 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVDFMRQSPLAGLDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT76901 NZ_CP021653:1877566-1877817 [Ralstonia solanacearum]
ATGCAAAGCTGGCAGTTGCAAGCCGCCAAGGCGCGGTTTTCCGACGTCGTGAAGCGCGCAGCGGACGATGGTCCGCAGGA
AATCACGGTGCATGGCCGCCCGGTCGCTGTTGTGATCTCGCGCGCACTGTTCGATCGCCTGAGTGGCGGCGGGGAGTCGC
TGGTCGACTTCATGCGCCAGTCTCCGTTGGCTGGCCTGGACGACGTCGTCTTCGAGCGCGAACGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA

Structures

Toxin

• AlphaFold DB: A0A399W071