Detailed information of TA system TA076897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/COG3657-dnstrm_HI1420
• Location: 65131..65741
• Replicon: plasmid unnamed
• Accession: NZ_CP021653
• Organism: Ralstonia solanacearum strain RS 488

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B5SAJ5
• Locus tag: CCY86_RS16305
• Protein ID: WP_003262835.1
• Coordinates: 65433..65741 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CCY86_RS16300
• Protein ID: WP_003262836.1
• Coordinates: 65131..65436 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCY86_RS16280	61238..61648	+	411	WP_003262840.1	acyl-CoA thioesterase	-
CCY86_RS16285	61678..62070	+	393	WP_003262838.1	RidA family protein	-
CCY86_RS16290	62418..63008	+	591	WP_020957579.1	hypothetical protein	-
CCY86_RS16295	63607..64920	-	1314	WP_097388942.1	MFS transporter	-
CCY86_RS16300	65131..65436	-	306	WP_003262836.1	putative addiction module antidote protein	Antitoxin
CCY86_RS16305	65433..65741	-	309	WP_003262835.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCY86_RS16310	65817..67016	-	1200	WP_003262834.1	MFS transporter	-
CCY86_RS16315	67144..67962	-	819	WP_039556864.1	GntR family transcriptional regulator	-
CCY86_RS16320	68132..68683	-	552	WP_039556867.1	helix-turn-helix transcriptional regulator	-
CCY86_RS16325	68816..69415	+	600	WP_039556871.1	GNAT family N-acetyltransferase	-
CCY86_RS16330	69681..70115	+	435	WP_003274507.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999547	1999547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (9-98)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11900.72 Da
• Isoelectric Point: 7.2450

>T76897 WP_003262835.1 NZ_CP021653:c65741-65433 [Ralstonia solanacearum]
MLTIRTTELFDDWFCSLRDKAAQRRIQVRIDRLQMGHLGDMKAVRDGIRELRIDHGPGYRLYFVQHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T76897 NZ_CP021653:c65741-65433 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAACTCTTTGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGTCACCTGGGCGACATGAAAGCGGTACGTGACGGCATCCGCGAGCTGCGGATCG
ATCATGGGCCGGGCTACCGCTTGTACTTCGTGCAGCACGGCGTCGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACACAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11038.72 Da
• Isoelectric Point: 8.0667

>AT76897 WP_003262836.1 NZ_CP021653:c65436-65131 [Ralstonia solanacearum]
MTMAQRTLKTRPWDSAEHLRTEADMAAYLNACLEEAGDDPAFITHALGVVARARGMTRLARETGITREGLYKALSEDGNP
SFATMLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT76897 NZ_CP021653:c65436-65131 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCCTCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAGGCGGACATGGCCGC
CTATCTCAACGCCTGCCTGGAAGAAGCCGGGGACGACCCGGCGTTCATTACGCACGCGCTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACCCGGCTCGCCCGCGAGACCGGGATCACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAACCCG
AGCTTTGCGACCATGCTGAAGGTCATCCGGGCACTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: B5SAJ5