Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 42291..42826 Replicon plasmid p2-T1
Accession NZ_CP021650
Organism Acidovorax sp. T1

Toxin (Protein)


Gene name mazF Uniprot ID A0A7W6J9K5
Locus tag CCX87_RS20080 Protein ID WP_021561444.1
Coordinates 42506..42826 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7W6J9M0
Locus tag CCX87_RS20075 Protein ID WP_021561443.1
Coordinates 42291..42506 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCX87_RS20040 37740..38327 + 588 WP_010890138.1 conjugal transfer protein TrbM -
CCX87_RS20045 38341..38976 + 636 WP_011114052.1 transglycosylase SLT domain-containing protein -
CCX87_RS20050 39005..39271 + 267 WP_010890140.1 hypothetical protein -
CCX87_RS20055 39271..39969 + 699 WP_011114053.1 conjugal transfer protein TraX -
CCX87_RS20060 39985..40416 + 432 WP_001665065.1 OmpA family protein -
CCX87_RS20065 40571..41245 + 675 WP_011178411.1 DNA methylase -
CCX87_RS20070 41247..41906 - 660 WP_011178412.1 recombinase family protein -
CCX87_RS20075 42291..42506 + 216 WP_021561443.1 antitoxin MazE family protein Antitoxin
CCX87_RS20080 42506..42826 + 321 WP_021561444.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CCX87_RS20085 43062..43625 + 564 WP_021561445.1 recombinase family protein -
CCX87_RS20095 43888..45080 + 1193 WP_087748592.1 IS3 family transposase -
CCX87_RS20100 45137..45352 + 216 WP_087748593.1 hypothetical protein -
CCX87_RS20105 45357..46157 - 801 WP_087748594.1 phosphonate ABC transporter, permease protein PhnE -
CCX87_RS20110 46180..47127 - 948 WP_087748595.1 phosphonate ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..79174 79174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin MazE-like

(7-70)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11585.51 Da        Isoelectric Point: 6.2890

>T76894 WP_021561444.1 NZ_CP021650:42506-42826 [Acidovorax sp. T1]
MQRGDLVTVSLQGDYGKPRPALIVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Download         Length: 321 bp

>T76894 NZ_CP021650:42506-42826 [Acidovorax sp. T1]
ATGCAGCGAGGCGACCTCGTGACGGTTTCATTGCAGGGTGACTACGGCAAACCACGACCAGCCCTGATCGTTCAATCAGA
CCTACTAACCGACTTGGAAAGCGTTGTGCTGTGCCCAGTGACAAGCGACCTGCGCAATGCCGCGTTTCGCGTCACCGTGG
AACCGAATCCAGCTAACGGGCTGCGCGCACTATCGCAAGTTATGGTGGACAAGCTCTCGACGCTACCGCGCACCAAAATC
AGTGAACCGTTTGGCCGCCTCGACGATGAGCGAATGAAAGCAATAGATAGGGCGCTCCTGTTGGTCGTTGGCGTTATTTG
A

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8159.19 Da        Isoelectric Point: 7.2801

>AT76894 WP_021561443.1 NZ_CP021650:42291-42506 [Acidovorax sp. T1]
MPSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFTAQVRKQCQSLKGDPAEAEVLRFTEEAASHVEGWE

Download         Length: 216 bp

>AT76894 NZ_CP021650:42291-42506 [Acidovorax sp. T1]
ATGCCATCAATTCACGAATCAAAGGTAGCCCGACACCGCGAACGGATGCGCGCGGCGGGGCTGCGGCCTGTGCAGTTTTG
GGTGCCGGACACCCGTTCGCCTGAGTTCACGGCGCAAGTGCGCAAGCAATGCCAAAGCCTGAAGGGCGACCCAGCAGAAG
CAGAGGTTTTGCGCTTCACCGAAGAGGCGGCATCGCACGTTGAGGGCTGGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W6J9K5

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6J9M0

References