Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1713928..1714508 Replicon chromosome
Accession NZ_CP021644
Organism Glaesserella parasuis 29755

Toxin (Protein)


Gene name mazF Uniprot ID A0A143CFE3
Locus tag B4U42_RS08955 Protein ID WP_005710599.1
Coordinates 1714164..1714508 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag B4U42_RS08950 Protein ID WP_005710601.1
Coordinates 1713928..1714167 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4U42_RS08925 1709632..1710324 - 693 WP_005710610.1 tellurite resistance TerB family protein -
B4U42_RS08930 1710386..1711234 - 849 WP_005710608.1 ABC transporter permease subunit -
B4U42_RS08935 1711231..1712016 - 786 WP_005710607.1 ABC transporter permease subunit -
B4U42_RS08940 1712006..1712758 - 753 WP_102033221.1 phosphonate ABC transporter ATP-binding protein -
B4U42_RS08945 1712867..1713742 - 876 WP_005710603.1 putative selenate ABC transporter substrate-binding protein -
B4U42_RS08950 1713928..1714167 + 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B4U42_RS08955 1714164..1714508 + 345 WP_005710599.1 endoribonuclease MazF Toxin
B4U42_RS08960 1714514..1715182 + 669 WP_005710597.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
B4U42_RS08965 1715195..1716586 + 1392 WP_005710595.1 MATE family efflux transporter -
B4U42_RS08970 1716658..1718763 - 2106 WP_005710593.1 hypothetical protein -
B4U42_RS08975 1718969..1719265 - 297 WP_005710591.1 DUF5374 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12830.81 Da        Isoelectric Point: 7.9277

>T76879 WP_005710599.1 NZ_CP021644:1714164-1714508 [Glaesserella parasuis 29755]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPTIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T76879 NZ_CP021644:1714164-1714508 [Glaesserella parasuis 29755]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGCCATCGTCCAGCATTAGTGTTAACGCCCACGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCGCTCACAA
CAAAAGTAAAAGGCTATCCTTTCGAAGTCAATATTGCAGGTACCCCCCAAAATGTTGTGCTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT76879 WP_005710601.1 NZ_CP021644:1713928-1714167 [Glaesserella parasuis 29755]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT76879 NZ_CP021644:1713928-1714167 [Glaesserella parasuis 29755]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCAGGTATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143CFE3

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References