Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1073305..1073941 Replicon chromosome
Accession NZ_CP021559
Organism Bifidobacterium breve strain CNCM I-4321

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag CNCMI4321_RS04885 Protein ID WP_003829234.1
Coordinates 1073305..1073661 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag CNCMI4321_RS04890 Protein ID WP_012577921.1
Coordinates 1073648..1073941 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNCMI4321_RS04855 1068495..1069406 + 912 WP_014483811.1 ABC transporter ATP-binding protein -
CNCMI4321_RS04860 1069603..1070229 + 627 WP_003829226.1 30S ribosomal protein S4 -
CNCMI4321_RS04865 1070568..1071350 - 783 WP_014483809.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
CNCMI4321_RS04870 1071488..1071973 - 486 WP_014483808.1 cytidine deaminase -
CNCMI4321_RS04875 1072065..1072421 + 357 WP_014483807.1 SdpI family protein -
CNCMI4321_RS04880 1072526..1073128 + 603 WP_003829233.1 transglutaminase family protein -
CNCMI4321_RS04885 1073305..1073661 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CNCMI4321_RS04890 1073648..1073941 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CNCMI4321_RS04895 1074092..1074256 - 165 Protein_916 IS30 family transposase -
CNCMI4321_RS04900 1074356..1075030 + 675 WP_015438748.1 histidine phosphatase family protein -
CNCMI4321_RS04905 1075113..1075517 + 405 WP_014483805.1 DUF948 domain-containing protein -
CNCMI4321_RS04910 1075517..1075753 + 237 WP_003829239.1 hypothetical protein -
CNCMI4321_RS04915 1075849..1078527 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76867 WP_003829234.1 NZ_CP021559:c1073661-1073305 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76867 NZ_CP021559:c1073661-1073305 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76867 WP_012577921.1 NZ_CP021559:c1073941-1073648 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76867 NZ_CP021559:c1073941-1073648 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References