Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1062367..1063003 Replicon chromosome
Accession NZ_CP021554
Organism Bifidobacterium breve strain 017W439

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag BB017W439_RS04645 Protein ID WP_003829234.1
Coordinates 1062367..1062723 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag BB017W439_RS04650 Protein ID WP_012577921.1
Coordinates 1062710..1063003 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB017W439_RS04615 1057556..1058467 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
BB017W439_RS04620 1058664..1059290 + 627 WP_003829226.1 30S ribosomal protein S4 -
BB017W439_RS04625 1059627..1060409 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
BB017W439_RS04630 1060551..1061036 - 486 WP_003829229.1 cytidine deaminase -
BB017W439_RS04635 1061127..1061483 + 357 WP_052788397.1 SdpI family protein -
BB017W439_RS04640 1061588..1062190 + 603 WP_052788396.1 transglutaminase family protein -
BB017W439_RS04645 1062367..1062723 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BB017W439_RS04650 1062710..1063003 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BB017W439_RS04655 1063255..1063929 + 675 WP_015438748.1 histidine phosphatase family protein -
BB017W439_RS04660 1064012..1064395 + 384 WP_106648759.1 DUF948 domain-containing protein -
BB017W439_RS04665 1064395..1064631 + 237 WP_003829239.1 hypothetical protein -
BB017W439_RS04670 1064727..1067405 + 2679 WP_003829240.1 alanine--tRNA ligase -
BB017W439_RS04675 1067414..1067866 + 453 WP_003829241.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76855 WP_003829234.1 NZ_CP021554:c1062723-1062367 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76855 NZ_CP021554:c1062723-1062367 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76855 WP_012577921.1 NZ_CP021554:c1063003-1062710 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76855 NZ_CP021554:c1063003-1062710 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References