Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1165306..1165942 Replicon chromosome
Accession NZ_CP021553
Organism Bifidobacterium breve strain DRBB28

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag DRBB28_RS05295 Protein ID WP_003829234.1
Coordinates 1165306..1165662 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag DRBB28_RS05300 Protein ID WP_012577921.1
Coordinates 1165649..1165942 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRBB28_RS05265 1160492..1161403 + 912 WP_025341717.1 ABC transporter ATP-binding protein -
DRBB28_RS05270 1161600..1162226 + 627 WP_003829226.1 30S ribosomal protein S4 -
DRBB28_RS05275 1162564..1163346 - 783 WP_103619852.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
DRBB28_RS05280 1163487..1163972 - 486 WP_003829229.1 cytidine deaminase -
DRBB28_RS05285 1164066..1164422 + 357 WP_003833231.1 SdpI family protein -
DRBB28_RS05290 1164527..1165129 + 603 WP_003829233.1 transglutaminase family protein -
DRBB28_RS05295 1165306..1165662 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DRBB28_RS05300 1165649..1165942 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DRBB28_RS05310 1166397..1167071 + 675 WP_015438748.1 histidine phosphatase family protein -
DRBB28_RS05315 1167154..1167558 + 405 WP_003829238.1 DUF948 domain-containing protein -
DRBB28_RS05320 1167558..1167794 + 237 WP_003829239.1 hypothetical protein -
DRBB28_RS05325 1167890..1170568 + 2679 WP_106642003.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76853 WP_003829234.1 NZ_CP021553:c1165662-1165306 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76853 NZ_CP021553:c1165662-1165306 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76853 WP_012577921.1 NZ_CP021553:c1165942-1165649 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76853 NZ_CP021553:c1165942-1165649 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References