Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1105518..1106073 Replicon chromosome
Accession NZ_CP021553
Organism Bifidobacterium breve strain DRBB28

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag DRBB28_RS05010 Protein ID WP_003829109.1
Coordinates 1105518..1105841 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag DRBB28_RS05015 Protein ID WP_003829112.1
Coordinates 1105828..1106073 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRBB28_RS04975 1100554..1100842 - 289 Protein_946 IS256 family transposase -
DRBB28_RS04985 1101313..1102578 - 1266 WP_106642002.1 Fic family protein -
DRBB28_RS04995 1103120..1103485 - 366 WP_065474609.1 YccF domain-containing protein -
DRBB28_RS05000 1103723..1104592 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
DRBB28_RS05005 1104687..1105301 + 615 WP_003829107.1 cupin domain-containing protein -
DRBB28_RS05010 1105518..1105841 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DRBB28_RS05015 1105828..1106073 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DRBB28_RS05020 1106247..1107503 + 1257 WP_003829116.1 HipA domain-containing protein -
DRBB28_RS05025 1107768..1107959 + 192 WP_025341701.1 XRE family transcriptional regulator -
DRBB28_RS05030 1108143..1109414 - 1272 WP_106629903.1 MFS transporter -
DRBB28_RS05035 1109571..1110233 - 663 WP_019728044.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1096173..1147247 51074


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T76852 WP_003829109.1 NZ_CP021553:c1105841-1105518 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76852 NZ_CP021553:c1105841-1105518 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76852 WP_003829112.1 NZ_CP021553:c1106073-1105828 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76852 NZ_CP021553:c1106073-1105828 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References