Detailed information of TA system TA076850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1124586..1125222
• Replicon: chromosome
• Accession: NZ_CP021552
• Organism: Bifidobacterium breve strain DRBB27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2K9BIJ9
• Locus tag: DRBB27_RS05205
• Protein ID: WP_032739732.1
• Coordinates: 1124586..1124942 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2K9C8D0
• Locus tag: DRBB27_RS05210
• Protein ID: WP_025221547.1
• Coordinates: 1124929..1125222 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRBB27_RS05170	1119904..1120530	+	627	WP_003829226.1	30S ribosomal protein S4	-
DRBB27_RS05175	1121005..1121787	-	783	WP_106621604.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DRBB27_RS05180	1121926..1122411	-	486	WP_025299774.1	cytidine deaminase	-
DRBB27_RS05185	1122501..1122857	+	357	WP_038426137.1	SdpI family protein	-
DRBB27_RS05190	1122962..1123564	+	603	WP_003829233.1	transglutaminase family protein	-
DRBB27_RS05195	1123571..1123958	+	388	Protein_980	linear amide C-N hydrolase	-
DRBB27_RS05200	1123992..1124417	+	426	WP_106621605.1	hypothetical protein	-
DRBB27_RS05205	1124586..1124942	-	357	WP_032739732.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DRBB27_RS05210	1124929..1125222	-	294	WP_025221547.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DRBB27_RS05215	1125369..1126445	+	1077	Protein_984	IS30 family transposase	-
DRBB27_RS05220	1126566..1126886	-	321	WP_016463217.1	hypothetical protein	-
DRBB27_RS05225	1126905..1127522	-	618	WP_157825946.1	hypothetical protein	-
DRBB27_RS05230	1127842..1129767	-	1926	WP_016463219.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1121926..1152525	30599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13244.21 Da
• Isoelectric Point: 9.5969

>T76850 WP_032739732.1 NZ_CP021552:c1124942-1124586 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDGGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T76850 NZ_CP021552:c1124942-1124586 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGGCGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10836.28 Da
• Isoelectric Point: 4.2896

>AT76850 WP_025221547.1 NZ_CP021552:c1125222-1124929 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT76850 NZ_CP021552:c1125222-1124929 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A2K9BIJ9

Antitoxin

• AlphaFold DB: A0A2K9C8D0