Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36399..37042 Replicon plasmid tig00000003
Accession NZ_CP021547
Organism Klebsiella pneumoniae strain AR_0112

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM359_RS02735 Protein ID WP_000754566.1
Coordinates 36399..36815 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM359_RS02740 Protein ID WP_001261276.1
Coordinates 36812..37042 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM359_RS02695 31907..32128 + 222 Protein_33 nucleotidyltransferase domain-containing protein -
AM359_RS02700 32188..32892 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM359_RS02710 32947..33117 + 171 Protein_35 serine hydrolase -
AM359_RS02720 33981..35057 - 1077 WP_000227969.1 IS110 family transposase -
AM359_RS02730 35339..36195 - 857 Protein_37 IS3-like element ISEc15 family transposase -
AM359_RS02735 36399..36815 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM359_RS02740 36812..37042 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM359_RS02750 37616..37966 + 351 WP_000493378.1 hypothetical protein -
AM359_RS02755 38017..38760 + 744 WP_000129823.1 hypothetical protein -
AM359_RS02760 38757..39533 + 777 WP_000015958.1 site-specific integrase -
AM359_RS02765 39591..39848 - 258 WP_000764642.1 hypothetical protein -
AM359_RS30435 39977..40081 - 105 WP_032409716.1 hypothetical protein -
AM359_RS02770 40611..41477 + 867 WP_004118283.1 replication initiation protein -
AM359_RS02775 41654..41923 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aac(6')-Ib - 1..45740 45740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T76826 WP_000754566.1 NZ_CP021547:c36815-36399 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T76826 NZ_CP021547:c36815-36399 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT76826 WP_001261276.1 NZ_CP021547:c37042-36812 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT76826 NZ_CP021547:c37042-36812 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References