Detailed information of TA system TA076825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 44054..44543
• Replicon: plasmid tig00000002
• Accession: NZ_CP021546
• Organism: Klebsiella pneumoniae strain AR_0112

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U3PL64
• Locus tag: AM359_RS02310
• Protein ID: WP_020314676.1
• Coordinates: 44054..44314 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: AM359_RS02315
• Protein ID: WP_022540736.1
• Coordinates: 44304..44543 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM359_RS02260	40142..40471	+	330	WP_001542022.1	hypothetical protein	-
AM359_RS02265	40875..41390	+	516	WP_001025393.1	J domain-containing protein	-
AM359_RS02270	41494..42036	+	543	WP_001083904.1	hypothetical protein	-
AM359_RS02275	42033..42287	+	255	WP_001230709.1	hypothetical protein	-
AM359_RS02280	42284..42526	+	243	WP_000151456.1	hypothetical protein	-
AM359_RS02295	43107..43430	+	324	WP_020314677.1	hypothetical protein	-
AM359_RS02300	43472..43681	+	210	WP_020314678.1	hypothetical protein	-
AM359_RS02305	43778..44002	+	225	WP_000814381.1	hypothetical protein	-
AM359_RS02310	44054..44314	+	261	WP_020314676.1	hypothetical protein	Toxin
AM359_RS02315	44304..44543	+	240	WP_022540736.1	transcriptional regulator	Antitoxin
AM359_RS02330	45078..45338	+	261	WP_020314977.1	hypothetical protein	-
AM359_RS02335	45725..45907	-	183	WP_001401700.1	hypothetical protein	-
AM359_RS02340	46452..46634	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM359_RS02345	46659..47096	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AM359_RS02350	47227..47547	-	321	WP_001310435.1	hypothetical protein	-
AM359_RS02355	47544..48059	-	516	WP_001229189.1	hypothetical protein	-
AM359_RS02360	48096..48359	-	264	WP_020314988.1	hypothetical protein	-
AM359_RS02365	48361..48780	-	420	WP_001708405.1	hypothetical protein	-
AM359_RS02370	48800..49507	-	708	WP_000108796.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaTEM-1A	-	1..74660	74660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9922.30 Da
• Isoelectric Point: 7.2028

>T76825 WP_020314676.1 NZ_CP021546:44054-44314 [Klebsiella pneumoniae]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRDNDKEIVFMMIQEMS
EEENAN

Nucleotide

• Length: 261 bp

>T76825 NZ_CP021546:44054-44314 [Klebsiella pneumoniae]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGATAATGATAAAGAAATTGTTTTTATGATGATTCAGGAAATGAGC
GAAGAAGAAAATGCTAACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9072.61 Da
• Isoelectric Point: 10.2613

>AT76825 WP_022540736.1 NZ_CP021546:44304-44543 [Klebsiella pneumoniae]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLLLLAGEHPEYVLSKRDRGILK

Nucleotide

• Length: 240 bp

>AT76825 NZ_CP021546:44304-44543 [Klebsiella pneumoniae]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGAAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACATGGCGTCGTAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCCGTGG
AGTTTAAATATCTTCTGCTTTTAGCTGGGGAACATCCTGAATACGTCCTTAGTAAAAGAGATCGAGGAATTTTAAAATGA

Structures

Toxin

• AlphaFold DB: A0A376MG86