Detailed information of TA system TA076824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 42033..42526
• Replicon: plasmid tig00000002
• Accession: NZ_CP021546
• Organism: Klebsiella pneumoniae strain AR_0112

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: AM359_RS02275
• Protein ID: WP_001230709.1
• Coordinates: 42033..42287 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: AM359_RS02280
• Protein ID: WP_000151456.1
• Coordinates: 42284..42526 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM359_RS02235	37607..37768	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
AM359_RS02240	37918..38514	+	597	WP_000125164.1	hypothetical protein	-
AM359_RS30425	38532..38816	-	285	WP_001162371.1	hypothetical protein	-
AM359_RS02250	38890..39555	-	666	WP_000532148.1	P-loop NTPase	-
AM359_RS02260	40142..40471	+	330	WP_001542022.1	hypothetical protein	-
AM359_RS02265	40875..41390	+	516	WP_001025393.1	J domain-containing protein	-
AM359_RS02270	41494..42036	+	543	WP_001083904.1	hypothetical protein	-
AM359_RS02275	42033..42287	+	255	WP_001230709.1	hypothetical protein	Toxin
AM359_RS02280	42284..42526	+	243	WP_000151456.1	hypothetical protein	Antitoxin
AM359_RS02295	43107..43430	+	324	WP_020314677.1	hypothetical protein	-
AM359_RS02300	43472..43681	+	210	WP_020314678.1	hypothetical protein	-
AM359_RS02305	43778..44002	+	225	WP_000814381.1	hypothetical protein	-
AM359_RS02310	44054..44314	+	261	WP_020314676.1	hypothetical protein	-
AM359_RS02315	44304..44543	+	240	WP_022540736.1	transcriptional regulator	-
AM359_RS02330	45078..45338	+	261	WP_020314977.1	hypothetical protein	-
AM359_RS02335	45725..45907	-	183	WP_001401700.1	hypothetical protein	-
AM359_RS02340	46452..46634	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM359_RS02345	46659..47096	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaTEM-1A	-	1..74660	74660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T76824 WP_001230709.1 NZ_CP021546:42033-42287 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T76824 NZ_CP021546:42033-42287 [Klebsiella pneumoniae]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT76824 WP_000151456.1 NZ_CP021546:42284-42526 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT76824 NZ_CP021546:42284-42526 [Klebsiella pneumoniae]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5