Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43102..43627 Replicon plasmid tig00000001
Accession NZ_CP021545
Organism Klebsiella pneumoniae strain AR_0112

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag AM359_RS01450 Protein ID WP_001568026.1
Coordinates 43322..43627 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AM359_RS01445 Protein ID WP_001568025.1
Coordinates 43102..43320 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM359_RS01400 38479..38682 - 204 WP_004197809.1 hypothetical protein -
AM359_RS01405 38696..38899 - 204 WP_004197808.1 hemolysin expression modulator Hha -
AM359_RS01410 38933..39301 - 369 WP_004197807.1 hypothetical protein -
AM359_RS01415 39345..39839 - 495 WP_020802777.1 hypothetical protein -
AM359_RS01420 39870..40445 - 576 WP_020317495.1 hypothetical protein -
AM359_RS01425 40433..40702 - 270 WP_020804424.1 hypothetical protein -
AM359_RS01430 41139..41828 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
AM359_RS01435 41860..42549 - 690 WP_004193995.1 hypothetical protein -
AM359_RS01445 43102..43320 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM359_RS01450 43322..43627 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
AM359_RS01455 43796..44191 + 396 WP_017899885.1 hypothetical protein -
AM359_RS01460 44218..44541 + 324 WP_022644730.1 hypothetical protein -
AM359_RS01465 44538..45554 + 1017 WP_020315256.1 hypothetical protein -
AM359_RS01470 45752..46546 + 795 WP_004197635.1 site-specific integrase -
AM359_RS01475 47002..47181 - 180 WP_006788217.1 hypothetical protein -
AM359_RS01480 47301..47927 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..125536 125536


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T76823 WP_001568026.1 NZ_CP021545:43322-43627 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T76823 NZ_CP021545:43322-43627 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT76823 WP_001568025.1 NZ_CP021545:43102-43320 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT76823 NZ_CP021545:43102-43320 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References