Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 21421..22157 Replicon plasmid tig00000000
Accession NZ_CP021544
Organism Klebsiella pneumoniae strain AR_0112

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM359_RS00155 Protein ID WP_003026803.1
Coordinates 21675..22157 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM359_RS00150 Protein ID WP_003026799.1
Coordinates 21421..21687 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM359_RS00105 17483..17845 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM359_RS00110 17895..18245 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM359_RS00115 18603..18872 + 270 WP_004152102.1 hypothetical protein -
AM359_RS00120 18860..19435 + 576 WP_004152103.1 hypothetical protein -
AM359_RS00125 19466..19960 + 495 WP_004152104.1 DNA-binding protein -
AM359_RS00130 20004..20372 + 369 WP_004152105.1 hypothetical protein -
AM359_RS00135 20406..20609 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AM359_RS00140 20658..20915 + 258 WP_004152107.1 hypothetical protein -
AM359_RS00145 20991..21245 + 255 WP_004152108.1 hypothetical protein -
AM359_RS00150 21421..21687 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM359_RS00155 21675..22157 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM359_RS00160 22369..23715 + 1347 WP_020314316.1 ISNCY family transposase -
AM359_RS00165 24216..25470 - 1255 Protein_32 IS3 family transposase -
AM359_RS00170 25558..26520 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..208223 208223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T76822 WP_003026803.1 NZ_CP021544:21675-22157 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T76822 NZ_CP021544:21675-22157 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT76822 WP_003026799.1 NZ_CP021544:21421-21687 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT76822 NZ_CP021544:21421-21687 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References