Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 5131..5665 Replicon plasmid unitig_4
Accession NZ_CP021538
Organism Escherichia coli strain AR_0119

Toxin (Protein)


Gene name relE Uniprot ID A0A6P1V8P2
Locus tag AM366_RS28545 Protein ID WP_000143805.1
Coordinates 5375..5665 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A387K2U4
Locus tag AM366_RS28540 Protein ID WP_032635065.1
Coordinates 5131..5385 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM366_RS28520 484..933 - 450 WP_032635063.1 hypothetical protein -
AM366_RS28525 1183..3012 - 1830 WP_032635064.1 hypothetical protein -
AM366_RS28530 3780..4175 + 396 WP_032635081.1 hypothetical protein -
AM366_RS28535 4262..4972 + 711 WP_025760373.1 tyrosine-type recombinase/integrase -
AM366_RS28540 5131..5385 + 255 WP_032635065.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
AM366_RS28545 5375..5665 + 291 WP_000143805.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM366_RS28550 5976..6575 - 600 WP_032635044.1 DUF2913 family protein -
AM366_RS28560 7381..7977 + 597 WP_032635043.1 recombinase family protein -
AM366_RS28565 8267..10264 + 1998 WP_032635075.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 - 1..64372 64372
- flank IS/Tn - - 7381..7977 596


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-47)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11463.41 Da        Isoelectric Point: 10.2689

>T76802 WP_000143805.1 NZ_CP021538:5375-5665 [Escherichia coli]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Download         Length: 291 bp

>T76802 NZ_CP021538:5375-5665 [Escherichia coli]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAACCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9722.07 Da        Isoelectric Point: 4.4356

>AT76802 WP_032635065.1 NZ_CP021538:5131-5385 [Escherichia coli]
MSTIQIRVDDELKKEAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Download         Length: 255 bp

>AT76802 NZ_CP021538:5131-5385 [Escherichia coli]
ATGAGCACAATTCAGATTCGCGTTGATGACGAGCTGAAAAAAGAGGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCAGACGCACTTCGTCTGTTCCTGCGATATGTTGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTGGTTCGGGAAAGACTGAAAAATCCTGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V8P2

Antitoxin

Source ID Structure
AlphaFold DB A0A387K2U4

References