Detailed information of TA system TA076800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 12279..12880
• Replicon: plasmid unitig_3
• Accession: NZ_CP021537
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: AM366_RS27970
• Protein ID: WP_053919810.1
• Coordinates: 12279..12659 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AM366_RS27975
• Protein ID: WP_001190712.1
• Coordinates: 12659..12880 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS27940	8286..9314	+	1029	WP_087758011.1	tyrosine-type recombinase/integrase	-
AM366_RS27945	9424..9732	+	309	WP_000349257.1	hypothetical protein	-
AM366_RS27950	9729..10205	+	477	WP_000861175.1	hypothetical protein	-
AM366_RS27955	10202..10696	+	495	WP_000640907.1	dUTP diphosphatase	-
AM366_RS27960	10711..11388	+	678	WP_087758012.1	DUF2829 domain-containing protein	-
AM366_RS27965	11395..12195	+	801	WP_087758013.1	hypothetical protein	-
AM366_RS27970	12279..12659	-	381	WP_053919810.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM366_RS27975	12659..12880	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM366_RS27980	12953..13342	-	390	WP_087758014.1	DNA repair protein	-
AM366_RS27990	13743..15230	+	1488	WP_162287626.1	group II intron reverse transcriptase/maturase	-
AM366_RS27995	15375..15626	-	252	WP_001377386.1	DNA polymerase III subunit theta	-
AM366_RS29850	16076..16213	+	138	WP_000123562.1	hypothetical protein	-
AM366_RS28010	16288..16650	-	363	WP_001261544.1	hypothetical protein	-
AM366_RS28015	16647..17579	-	933	WP_000057449.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97474	97474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13662.55 Da
• Isoelectric Point: 5.6406

>T76800 WP_053919810.1 NZ_CP021537:c12659-12279 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T76800 NZ_CP021537:c12659-12279 [Escherichia coli]
ATGAGGCATATATCACCAGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTAGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGCATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT76800 WP_001190712.1 NZ_CP021537:c12880-12659 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT76800 NZ_CP021537:c12880-12659 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6