Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 214550..215075 Replicon plasmid unitig_2
Accession NZ_CP021536
Organism Escherichia coli strain AR_0119

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AM366_RS27695 Protein ID WP_001159868.1
Coordinates 214550..214855 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AM366_RS27700 Protein ID WP_000813634.1
Coordinates 214857..215075 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM366_RS27680 210460..211626 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
AM366_RS27685 212214..212969 - 756 WP_000852146.1 replication initiation protein RepE -
AM366_RS27690 213743..214549 - 807 WP_000016982.1 site-specific integrase -
AM366_RS27695 214550..214855 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AM366_RS27700 214857..215075 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM366_RS27705 215783..216778 + 996 WP_000246636.1 hypothetical protein -
AM366_RS27710 216782..217714 + 933 WP_058682609.1 hypothetical protein -
AM366_RS27715 217914..218611 - 698 Protein_275 IS1 family transposase -
AM366_RS27725 218669..218917 + 249 Protein_276 abortive infection family protein -
AM366_RS27730 219206..220018 - 813 WP_016236297.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / qacE / sul1 / blaNDM-1 / rmtC / blaCTX-M-15 / aac(3)-IIa / blaTEM-1B / blaCMY-6 htpB 1..244955 244955
- flank IS/Tn - - 217914..218291 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T76799 WP_001159868.1 NZ_CP021536:c214855-214550 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T76799 NZ_CP021536:c214855-214550 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT76799 WP_000813634.1 NZ_CP021536:c215075-214857 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT76799 NZ_CP021536:c215075-214857 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References