Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4093954..4094572 Replicon chromosome
Accession NZ_CP021535
Organism Escherichia coli strain AR_0119

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM366_RS21330 Protein ID WP_001291435.1
Coordinates 4094354..4094572 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM366_RS21325 Protein ID WP_000344800.1
Coordinates 4093954..4094328 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM366_RS21315 4089043..4090236 + 1194 WP_087757988.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM366_RS21320 4090259..4093408 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM366_RS21325 4093954..4094328 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM366_RS21330 4094354..4094572 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM366_RS21335 4094743..4095294 + 552 WP_000102564.1 maltose O-acetyltransferase -
AM366_RS21340 4095410..4095880 + 471 WP_000136192.1 YlaC family protein -
AM366_RS21345 4096044..4097594 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM366_RS21350 4097636..4097989 - 354 WP_000878141.1 DUF1428 family protein -
AM366_RS21360 4098368..4098679 + 312 WP_000409911.1 MGMT family protein -
AM366_RS21365 4098710..4099282 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T76786 WP_001291435.1 NZ_CP021535:4094354-4094572 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T76786 NZ_CP021535:4094354-4094572 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT76786 WP_000344800.1 NZ_CP021535:4093954-4094328 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT76786 NZ_CP021535:4093954-4094328 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References