Detailed information of TA system TA076782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3044642..3045280
• Replicon: chromosome
• Accession: NZ_CP021535
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AM366_RS15795
• Protein ID: WP_000813794.1
• Coordinates: 3045104..3045280 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM366_RS15790
• Protein ID: WP_001270286.1
• Coordinates: 3044642..3045058 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS15770	3039794..3040735	-	942	WP_001251315.1	ABC transporter permease	-
AM366_RS15775	3040736..3041749	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AM366_RS15780	3041767..3042912	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AM366_RS15785	3043157..3044563	-	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
AM366_RS15790	3044642..3045058	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM366_RS15795	3045104..3045280	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM366_RS15800	3045502..3045732	+	231	WP_000494244.1	YncJ family protein	-
AM366_RS15805	3045824..3047785	-	1962	WP_001593323.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM366_RS15810	3047858..3048394	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AM366_RS15815	3048447..3049661	+	1215	WP_001348055.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3049701..3050849	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T76782 WP_000813794.1 NZ_CP021535:c3045280-3045104 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T76782 NZ_CP021535:c3045280-3045104 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT76782 WP_001270286.1 NZ_CP021535:c3045058-3044642 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT76782 NZ_CP021535:c3045058-3044642 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB