Detailed information of TA system TA076739

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1417479..1418173
• Replicon: chromosome
• Accession: NZ_CP021532
• Organism: Escherichia coli strain AR_0149

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: AM396_RS07095
• Protein ID: WP_001094399.1
• Coordinates: 1417805..1418173 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM396_RS07090
• Protein ID: WP_077249034.1
• Coordinates: 1417479..1417784 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM396_RS07045	1413433..1413888	+	456	WP_000581506.1	hypothetical protein	-
AM396_RS25455	1413989..1414197	+	209	Protein_1275	DUF905 family protein	-
AM396_RS07055	1414298..1415119	+	822	WP_001761104.1	DUF945 domain-containing protein	-
AM396_RS07065	1415461..1415934	+	474	WP_072647970.1	antirestriction protein	-
AM396_RS07070	1415950..1416426	+	477	WP_001186773.1	RadC family protein	-
AM396_RS07075	1416489..1416710	+	222	WP_000692315.1	DUF987 domain-containing protein	-
AM396_RS25460	1416755..1417375	+	621	WP_106475231.1	antitoxin of toxin-antitoxin stability system	-
AM396_RS07090	1417479..1417784	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM396_RS07095	1417805..1418173	+	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
AM396_RS07100	1418494..1418832	-	339	Protein_1283	LysR family transcriptional regulator	-
AM396_RS07105	1418883..1419839	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM396_RS07110	1419849..1422047	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM396_RS07115	1422044..1423000	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T76739 WP_001094399.1 NZ_CP021532:1417805-1418173 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T76739 NZ_CP021532:1417805-1418173 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT76739 WP_077249034.1 NZ_CP021532:1417479-1417784 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT76739 NZ_CP021532:1417479-1417784 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9