Detailed information of TA system TA076733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 34241..34920
• Replicon: plasmid pAP1447-2
• Accession: NZ_CP021526
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: S101447_RS15475
• Protein ID: WP_087636640.1
• Coordinates: 34241..34651 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JIL2
• Locus tag: S101447_RS15480
• Protein ID: WP_006115552.1
• Coordinates: 34648..34920 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS15440	29816..30637	+	822	WP_087636646.1	hypothetical protein	-
S101447_RS15445	30754..31602	+	849	WP_087636635.1	hypothetical protein	-
S101447_RS15450	31605..32069	+	465	WP_087636636.1	hypothetical protein	-
S101447_RS15455	32166..32981	+	816	WP_087636637.1	hypothetical protein	-
S101447_RS15460	32994..33398	+	405	WP_087636638.1	hypothetical protein	-
S101447_RS15465	33455..33775	-	321	WP_204251391.1	hypothetical protein	-
S101447_RS15470	33805..34005	-	201	WP_087636639.1	hypothetical protein	-
S101447_RS15475	34241..34651	-	411	WP_087636640.1	type II toxin-antitoxin system VapC family toxin	Toxin
S101447_RS15480	34648..34920	-	273	WP_006115552.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
S101447_RS15485	34993..35409	-	417	WP_087636641.1	hypothetical protein	-
S101447_RS15490	35406..35840	-	435	WP_087636539.1	plasmid protein	-
S101447_RS15495	35904..36401	-	498	WP_087636540.1	single-stranded DNA-binding protein	-
S101447_RS15825	37162..37308	-	147	WP_157894002.1	hypothetical protein	-
S101447_RS15830	37307..37474	+	168	WP_157893992.1	hypothetical protein	-
S101447_RS15505	37658..38338	-	681	WP_019089472.1	hypothetical protein	-
S101447_RS15835	38337..38609	+	273	WP_150178215.1	hypothetical protein	-
S101447_RS15510	38890..39243	+	354	WP_150178214.1	hypothetical protein	-
S101447_RS15515	39239..39623	-	385	Protein_49	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44181	44181
	-	flank	IS/Tn	-	-	39405..39623	218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14912.21 Da
• Isoelectric Point: 4.7237

>T76733 WP_087636640.1 NZ_CP021526:c34651-34241 [Acetobacter ascendens]
VSIYLLDTNIISDLVRNPSGSCAQRIEHIDPGDLCTSIIVAAELRYGCAKKGSEKLLNRVESVLAFIPVLPLDIPADCEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMRIRSLKVENWLEDVP

Nucleotide

• Length: 411 bp

>T76733 NZ_CP021526:c34651-34241 [Acetobacter ascendens]
GTGAGCATCTACCTTCTTGATACAAACATCATCAGCGATCTCGTGCGCAATCCTTCCGGCTCCTGTGCGCAACGCATTGA
GCATATTGACCCAGGTGATCTTTGCACCAGCATCATCGTGGCCGCAGAATTACGCTATGGATGCGCCAAAAAAGGCTCCG
AAAAGCTCTTGAACCGGGTCGAAAGCGTGTTGGCGTTTATCCCTGTGCTGCCACTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAAGCGGCAGGGCAGACAATCGGCATGAATGATCTGCTGATCGCGGCTCATGCTTACAC
ACTGAAAGCGACCCTGGTCACAAACAACACACGTGAGTTTATGCGCATCAGAAGCTTGAAGGTTGAGAACTGGCTGGAGG
ATGTGCCGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10440.04 Da
• Isoelectric Point: 4.5749

>AT76733 WP_006115552.1 NZ_CP021526:c34920-34648 [Acetobacter ascendens]
MPVPEVTPRPREVKLFRNNRSQAVRIPVEFEMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLPPEDDFPDIQ
DVAARPEDIL

Nucleotide

• Length: 273 bp

>AT76733 NZ_CP021526:c34920-34648 [Acetobacter ascendens]
ATGCCCGTTCCTGAAGTGACCCCCAGACCGCGCGAAGTAAAACTCTTTCGGAATAATCGCAGTCAGGCTGTGCGAATTCC
GGTCGAATTTGAAATGCCGGGAGATCGTGTGCTGATCCGCCGTGACGGTGACAAACTCGTTCTGGAGCCTGTGAAGACGC
CTTCCACGCTGAAGGAGCTTCTCATGGCATGGCGTGAAGAGCCGCAGCTGCCTCCCGAAGACGATTTTCCCGATATTCAG
GATGTTGCCGCCAGACCAGAAGATATCCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A401X8Q2