Detailed information of TA system TA076732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 313861..314390
• Replicon: plasmid pAP1447-1
• Accession: NZ_CP021525
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: S101447_RS15160
• Protein ID: WP_087636598.1
• Coordinates: 314100..314390 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: S101447_RS15155
• Protein ID: WP_087636597.1
• Coordinates: 313861..314103 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS15135	309657..311582	+	1926	WP_087636595.1	hypothetical protein	-
S101447_RS15140	311585..311821	+	237	WP_003627225.1	exodeoxyribonuclease VII small subunit	-
S101447_RS15145	311784..312377	-	594	WP_157894007.1	hypothetical protein	-
S101447_RS15150	312420..313504	-	1085	Protein_320	IS3 family transposase	-
S101447_RS15155	313861..314103	+	243	WP_087636597.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
S101447_RS15160	314100..314390	+	291	WP_087636598.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
S101447_RS15165	314393..315478	+	1086	WP_087636599.1	hypothetical protein	-
S101447_RS15170	315491..316537	-	1047	WP_087636600.1	Fic family protein	-
S101447_RS15175	316534..316890	-	357	WP_193562012.1	hypothetical protein	-
S101447_RS15180	316982..318276	-	1295	Protein_326	replication protein C	-
S101447_RS15190	319046..319324	-	279	WP_019088706.1	DUF2312 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336151	336151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10942.62 Da
• Isoelectric Point: 9.6563

>T76732 WP_087636598.1 NZ_CP021525:314100-314390 [Acetobacter ascendens]
VSRYALHFDPRAKREWDSLDGSIKILLKKALAKRLDEPHVSSAELSGDLRNCYKIKLRSSGYRLVYQMDDGKLIVLVIAI
GRRDAYEVYGDANCRA

Nucleotide

• Length: 291 bp

>T76732 NZ_CP021525:314100-314390 [Acetobacter ascendens]
GTGAGTCGATACGCGCTGCATTTCGACCCACGCGCCAAACGCGAGTGGGATAGTCTTGATGGAAGCATCAAGATACTGCT
CAAGAAAGCGCTGGCCAAAAGATTGGACGAACCACATGTTTCGTCAGCCGAACTTTCCGGGGACTTACGGAACTGTTACA
AAATCAAGCTTCGCTCATCTGGGTATCGTCTGGTGTACCAAATGGACGATGGTAAGCTTATTGTATTAGTCATCGCTATC
GGCCGCAGGGATGCCTATGAGGTTTATGGCGATGCAAACTGCCGTGCCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8949.36 Da
• Isoelectric Point: 5.0807

>AT76732 WP_087636597.1 NZ_CP021525:313861-314103 [Acetobacter ascendens]
MALINLRIDGSLKKAAYERLSELGVTPSDLIRQTFEYVVKTGKLPVRRTVISEEDQQLLAVVRERLASPEPSIPVSLDDL

Nucleotide

• Length: 243 bp

>AT76732 NZ_CP021525:313861-314103 [Acetobacter ascendens]
ATGGCTTTAATTAACCTGCGTATTGATGGCAGCCTAAAGAAAGCTGCTTACGAGCGGCTCTCTGAACTTGGCGTGACACC
GTCAGATCTCATCCGCCAGACGTTTGAATATGTTGTGAAAACAGGCAAGCTTCCCGTTCGGCGTACAGTTATCTCAGAAG
AGGATCAGCAACTGCTGGCGGTTGTACGTGAACGTCTTGCCTCTCCCGAACCTTCTATTCCCGTCTCGCTGGATGATCTG
TGA