Detailed information of TA system TA076731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 244135..244685
• Replicon: plasmid pAP1447-1
• Accession: NZ_CP021525
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7JII7
• Locus tag: S101447_RS14810
• Protein ID: WP_003618711.1
• Coordinates: 244135..244428 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D5QDQ9
• Locus tag: S101447_RS14815
• Protein ID: WP_003618686.1
• Coordinates: 244425..244685 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS14785	239937..240590	+	654	WP_087636619.1	arsenical resistance protein ArsH	-
S101447_RS14795	240642..241852	+	1211	WP_087636566.1	IS3 family transposase	-
S101447_RS14800	242058..242741	-	684	WP_019092458.1	DUF1275 domain-containing protein	-
S101447_RS14805	242741..244003	-	1263	WP_019092457.1	sodium:proton antiporter	-
S101447_RS14810	244135..244428	-	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
S101447_RS14815	244425..244685	-	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
S101447_RS14820	244919..245695	-	777	WP_019092456.1	SDR family oxidoreductase	-
S101447_RS14825	245768..246355	-	588	WP_087636567.1	DNA-3-methyladenine glycosylase I	-
S101447_RS14830	246376..247401	-	1026	WP_087636568.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
S101447_RS14835	247391..247792	-	402	WP_019092361.1	VOC family protein	-
S101447_RS14840	248011..248901	+	891	WP_019092362.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336151	336151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11605.35 Da
• Isoelectric Point: 7.9998

>T76731 WP_003618711.1 NZ_CP021525:c244428-244135 [Acetobacter ascendens]
MKEYCLEFDDRALREWDALDGSVRKKFEKKLEKLIWNPHSPGNELHRDLSGFYKIKLLKDGYRLVYQVIDERIVIYVIAV
GRRADNEIYELASKRTE

Nucleotide

• Length: 294 bp

>T76731 NZ_CP021525:c244428-244135 [Acetobacter ascendens]
ATGAAAGAGTATTGTCTTGAATTCGACGACAGAGCACTCAGAGAGTGGGATGCTCTTGACGGCAGCGTGCGTAAGAAATT
TGAAAAGAAGCTGGAAAAGCTGATCTGGAACCCTCACTCTCCAGGAAACGAACTGCATCGCGACCTATCAGGGTTTTATA
AGATCAAGCTTCTGAAAGATGGTTACCGCCTTGTTTATCAGGTCATCGACGAACGGATCGTCATCTATGTAATTGCCGTT
GGCCGGAGAGCCGATAATGAGATTTATGAACTGGCTTCGAAGCGAACAGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9762.23 Da
• Isoelectric Point: 10.4645

>AT76731 WP_003618686.1 NZ_CP021525:c244685-244425 [Acetobacter ascendens]
MSAVTFRVDETLKAAAVKKLSAQGISLSDALRDTLAYIAETGRSPVKRRLVTDEDAELIEIVRERLKNPAQKHRMTFAEL
KNRHRE

Nucleotide

• Length: 261 bp

>AT76731 NZ_CP021525:c244685-244425 [Acetobacter ascendens]
ATGTCTGCTGTTACATTCCGGGTAGATGAAACGCTGAAGGCAGCCGCAGTTAAGAAGTTGTCGGCTCAAGGCATTTCACT
TTCTGACGCACTGCGCGATACGCTCGCGTATATTGCTGAAACGGGTCGCTCTCCCGTCAAGCGGAGGCTTGTCACAGACG
AGGATGCGGAGCTTATCGAGATTGTGCGCGAACGCCTAAAAAACCCTGCCCAAAAGCATCGTATGACTTTTGCAGAGCTT
AAGAACCGTCATCGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T9M6