Detailed information of TA system TA076729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/VapC-MazE
• Location: 144382..145043
• Replicon: plasmid pAP1447-1
• Accession: NZ_CP021525
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A401XH43
• Locus tag: S101447_RS14270
• Protein ID: WP_019089356.1
• Coordinates: 144735..145043 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: S101447_RS14265
• Protein ID: WP_019089355.1
• Coordinates: 144382..144636 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS14225	139806..140627	+	822	WP_087636536.1	hypothetical protein	-
S101447_RS14230	140743..141591	+	849	WP_087636537.1	hypothetical protein	-
S101447_RS14235	141594..142058	+	465	WP_019089349.1	hypothetical protein	-
S101447_RS14240	142155..142970	+	816	WP_019089350.1	hypothetical protein	-
S101447_RS14245	142983..143387	+	405	WP_019089351.1	hypothetical protein	-
S101447_RS14250	143444..143764	-	321	WP_026019385.1	hypothetical protein	-
S101447_RS14255	143794..143994	-	201	WP_019089353.1	hypothetical protein	-
S101447_RS14260	144051..144380	-	330	WP_019089354.1	endoribonuclease MazF	-
S101447_RS14265	144382..144636	-	255	WP_019089355.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
S101447_RS14270	144735..145043	-	309	WP_019089356.1	PIN domain-containing protein	Toxin
S101447_RS14275	145040..145312	-	273	WP_019089357.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
S101447_RS14280	145385..145801	-	417	WP_087636538.1	hypothetical protein	-
S101447_RS14285	145798..146232	-	435	WP_087636539.1	plasmid protein	-
S101447_RS14290	146296..146793	-	498	WP_087636540.1	single-stranded DNA-binding protein	-
S101447_RS15770	147554..147700	-	147	WP_157894002.1	hypothetical protein	-
S101447_RS15775	147699..147866	+	168	WP_157893992.1	hypothetical protein	-
S101447_RS14300	148050..148730	-	681	WP_019089472.1	hypothetical protein	-
S101447_RS15780	148729..149001	+	273	WP_150178215.1	hypothetical protein	-
S101447_RS14305	149282..149635	+	354	WP_150178214.1	hypothetical protein	-
S101447_RS14310	149631..150015	-	385	Protein_157	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336151	336151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-76)

Antitoxin

• SpoVT-AbrB domain (10-50)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11094.83 Da
• Isoelectric Point: 4.3321

>T76729 WP_019089356.1 NZ_CP021525:c145043-144735 [Acetobacter ascendens]
VSIYLLDTNISSDFVRNPSGSCAQRIELIDPDDLCTSIIVAAELRYGCAKKGSEKLLNRVESVLAFIPVLILDIPADCEY
GGIRAELEAAGRTIGMLEKFFA

Nucleotide

• Length: 309 bp

>T76729 NZ_CP021525:c145043-144735 [Acetobacter ascendens]
GTGAGCATCTACCTTCTTGATACAAACATCAGCAGCGATTTCGTGCGCAACCCTTCCGGCTCCTGTGCACAACGCATTGA
GCTTATTGACCCAGATGATCTTTGCACCAGCATCATCGTGGCCGCAGAATTACGCTATGGATGCGCCAAAAAAGGCTCCG
AAAAGCTCTTGAACCGGGTCGAAAGCGTGTTGGCGTTTATCCCTGTGCTGATACTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAAGCAGCAGGGCGGACAATCGGCATGCTGGAGAAGTTTTTTGCGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8992.36 Da
• Isoelectric Point: 4.2950

>AT76729 WP_019089355.1 NZ_CP021525:c144636-144382 [Acetobacter ascendens]
MEDAMKAVVKKWGNSASIRIPASVLAAAGLKLDQPVDVREESGRIVIEPVTVVEYDLADLLAGITPENIHPEVDFGAPVG
RELL

Nucleotide

• Length: 255 bp

>AT76729 NZ_CP021525:c144636-144382 [Acetobacter ascendens]
ATGGAGGACGCAATGAAGGCTGTGGTAAAAAAATGGGGGAATAGCGCATCCATTCGTATCCCAGCTTCAGTATTAGCCGC
GGCTGGATTGAAGTTGGATCAGCCAGTGGATGTGCGCGAGGAAAGCGGACGCATTGTCATTGAGCCGGTCACAGTCGTAG
AATATGATCTAGCGGATTTGTTGGCTGGGATTACCCCGGAGAATATTCATCCTGAAGTGGACTTCGGAGCGCCTGTTGGC
CGCGAACTCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A401XH43