Detailed information of TA system TA076727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 90350..90852
• Replicon: plasmid pAP1447-1
• Accession: NZ_CP021525
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: S101447_RS13965
• Protein ID: WP_087636510.1
• Coordinates: 90589..90852 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: S101447_RS13960
• Protein ID: WP_087636509.1
• Coordinates: 90350..90592 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS13945	86175..88163	+	1989	WP_087636506.1	HAD-IA family hydrolase	-
S101447_RS13950	88259..89191	-	933	WP_157893998.1	sulfotransferase	-
S101447_RS13955	89570..90004	-	435	WP_087636508.1	MucR family transcriptional regulator	-
S101447_RS13960	90350..90592	+	243	WP_087636509.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
S101447_RS13965	90589..90852	+	264	WP_087636510.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
S101447_RS13970	90855..91940	+	1086	WP_087636511.1	hypothetical protein	-
S101447_RS13975	92090..92647	-	558	WP_087636512.1	CdiI family contact-dependent growth inhibition immunity protein	-
S101447_RS13980	92650..93888	-	1239	WP_087636610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336151	336151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (21-75)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10102.49 Da
• Isoelectric Point: 9.3723

>T76727 WP_087636510.1 NZ_CP021525:90589-90852 [Acetobacter ascendens]
MSRYALYFAPRAKREWGSLDGSIKRLDEPHVSSAEISRDLRDCYKIKLRSSGYRLVYQVDDGKLIVLVIALGRRDANEVY
DDTNHRI

Nucleotide

• Length: 264 bp

>T76727 NZ_CP021525:90589-90852 [Acetobacter ascendens]
TTGAGTCGATACGCGCTGTATTTCGCCCCACGCGCCAAACGCGAGTGGGGTAGTCTTGATGGAAGCATCAAGAGACTAGA
CGAACCACATGTTTCGTCAGCCGAAATTTCCAGGGACTTACGGGACTGTTACAAAATCAAGCTTCGCTCATCCGGGTATC
GTCTGGTGTACCAAGTGGACGATGGTAAGCTTATTGTATTAGTCATCGCTCTTGGCCGCAGGGATGCCAATGAAGTTTAT
GACGATACAAACCACCGTATCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9011.35 Da
• Isoelectric Point: 4.7719

>AT76727 WP_087636509.1 NZ_CP021525:90350-90592 [Acetobacter ascendens]
MASINLRIDDSLKKAAYERLSELSVTPSDLIRQTFEYVVKTGKLPVRRTVISEEDQQLLAVVRERLASPEPSIPVSLDDL

Nucleotide

• Length: 243 bp

>AT76727 NZ_CP021525:90350-90592 [Acetobacter ascendens]
ATGGCTTCAATTAACCTGCGTATTGATGACAGCCTAAAGAAAGCTGCTTACGAGCGGCTCTCTGAACTTAGCGTGACACC
GTCAGATCTCATCCGCCAGACGTTTGAGTATGTTGTGAAAACAGGCAAGCTTCCCGTTCGGCGTACAGTTATATCAGAAG
AGGATCAGCAACTGCTGGCGGTTGTACGTGAACGTCTTGCCTCTCCCGAACCTTCTATTCCCGTCTCGCTGGATGATCTT
TGA