Detailed information of TA system TA076726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2870666..2871240
• Replicon: chromosome
• Accession: NZ_CP021524
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2G4RF69
• Locus tag: S101447_RS13360
• Protein ID: WP_035365565.1
• Coordinates: 2871052..2871240 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: S101447_RS13355
• Protein ID: WP_087636328.1
• Coordinates: 2870666..2871055 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS15885	2866517..2866786	+	270	WP_087636448.1	hypothetical protein	-
S101447_RS13325	2866735..2867226	+	492	WP_087636324.1	hypothetical protein	-
S101447_RS13330	2867219..2868205	-	987	WP_087636325.1	FRG domain-containing protein	-
S101447_RS13335	2868337..2868747	+	411	WP_087636326.1	hypothetical protein	-
S101447_RS13340	2868897..2869656	+	760	Protein_2573	tyrosine-type recombinase/integrase	-
S101447_RS13345	2869694..2870326	+	633	WP_003628645.1	DUF3164 family protein	-
S101447_RS13350	2870323..2870652	+	330	WP_087636327.1	HNH endonuclease	-
S101447_RS13355	2870666..2871055	-	390	WP_087636328.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
S101447_RS13360	2871052..2871240	-	189	WP_035365565.1	type II toxin-antitoxin system HicA family toxin	Toxin
S101447_RS13365	2871458..2872060	+	603	WP_087636329.1	terminase	-
S101447_RS13370	2872044..2873735	+	1692	WP_087636330.1	terminase large subunit	-
S101447_RS13375	2873723..2875033	+	1311	WP_087636331.1	phage portal protein	-
S101447_RS13380	2875040..2875783	+	744	WP_087636332.1	Clp protease ClpP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2858373..2886498	28125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-119)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7018.25 Da
• Isoelectric Point: 11.1497

>T76726 WP_035365565.1 NZ_CP021524:c2871240-2871052 [Acetobacter ascendens]
MDSREIIKKIKADGWYLVATKGSHQQFKHPTKPGRVTVPHPKRDLPLGTLRSVEKQSGVKLK

Nucleotide

• Length: 189 bp

>T76726 NZ_CP021524:c2871240-2871052 [Acetobacter ascendens]
ATGGACAGCAGAGAGATCATCAAGAAGATCAAGGCAGACGGCTGGTATCTTGTCGCCACGAAAGGCAGTCATCAGCAGTT
CAAGCACCCGACAAAACCCGGCCGGGTGACGGTGCCTCACCCTAAGCGGGATCTTCCACTCGGCACTCTGCGGAGCGTAG
AGAAACAATCAGGGGTCAAACTGAAATGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14050.94 Da
• Isoelectric Point: 4.7313

>AT76726 WP_087636328.1 NZ_CP021524:c2871055-2870666 [Acetobacter ascendens]
MSDYIAIIHKEPESDFGVSFPDFPGCITAGSSLEEARKMAQEALEFHISGMTEDGESIPAPSPLDKVMQDPNFAQGVAFM
VHVRLPSKAVRVNVTLDESLVSAIAAVSKNRSKFLAEAAWEKLNRNQSV

Nucleotide

• Length: 390 bp

>AT76726 NZ_CP021524:c2871055-2870666 [Acetobacter ascendens]
ATGAGCGACTATATCGCCATCATCCACAAAGAGCCCGAAAGTGATTTCGGGGTCAGCTTTCCAGATTTTCCCGGCTGCAT
CACAGCAGGCTCTTCATTGGAAGAAGCCCGGAAAATGGCCCAGGAAGCCTTGGAATTCCATATCTCTGGCATGACAGAAG
ATGGTGAGTCCATCCCTGCGCCATCGCCTTTGGATAAGGTCATGCAGGATCCGAACTTTGCTCAAGGTGTTGCCTTCATG
GTTCATGTTCGGCTGCCTTCCAAAGCCGTGCGTGTGAACGTCACGTTGGACGAAAGCCTTGTGTCCGCCATTGCAGCTGT
CAGCAAAAACCGCTCCAAATTTCTGGCTGAAGCCGCATGGGAAAAACTGAACCGTAATCAGTCTGTATAA

Structures

Toxin

• AlphaFold DB: A0A2G4RF69