Detailed information of TA system TA076724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1918715..1919350
• Replicon: chromosome
• Accession: NZ_CP021524
• Organism: Acetobacter ascendens strain SRCM101447

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: S101447_RS09230
• Protein ID: WP_087635925.1
• Coordinates: 1918940..1919350 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G2I1D7
• Locus tag: S101447_RS09225
• Protein ID: WP_010511771.1
• Coordinates: 1918715..1918939 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101447_RS15865	1914540..1914725	-	186	WP_162288300.1	hypothetical protein	-
S101447_RS09210	1915594..1916193	+	600	WP_087635922.1	hypothetical protein	-
S101447_RS09215	1916543..1918072	+	1530	WP_162288301.1	recombinase family protein	-
S101447_RS09220	1918110..1918442	-	333	WP_157893978.1	hypothetical protein	-
S101447_RS09225	1918715..1918939	+	225	WP_010511771.1	hypothetical protein	Antitoxin
S101447_RS09230	1918940..1919350	+	411	WP_087635925.1	type II toxin-antitoxin system VapC family toxin	Toxin
S101447_RS09235	1919783..1922440	+	2658	WP_087635926.1	Hint domain-containing protein	-
S101447_RS09240	1922562..1922951	+	390	WP_070323530.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1877870..1919350	41480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (9-55)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15588.88 Da
• Isoelectric Point: 6.4680

>T76724 WP_087635925.1 NZ_CP021524:1918940-1919350 [Acetobacter ascendens]
MLRYLLDTNLCIRVLRDRPQGLRPRFNSCAEELCISDVVLYELLYGAERSSDPVRTRREVERFAARLAVLPFDSEAAAHT
ADIRAELERNGRIIGPYDLMIAGHARSRGLIVVTGNLREFQRVEGLRTEDWLTDMV

Nucleotide

• Length: 411 bp

>T76724 NZ_CP021524:1918940-1919350 [Acetobacter ascendens]
ATGTTGCGTTACCTGCTGGATACGAATCTCTGTATCCGGGTATTGCGGGATCGGCCGCAGGGGTTGCGTCCGAGATTCAA
CAGCTGTGCGGAGGAACTCTGCATCTCGGACGTGGTTCTGTATGAGCTGCTTTATGGCGCAGAACGCTCGTCCGATCCCG
TGCGCACAAGGCGTGAAGTCGAGCGCTTCGCGGCAAGGCTGGCGGTCCTGCCATTCGACAGTGAAGCAGCCGCCCATACG
GCAGACATTCGGGCCGAACTTGAACGGAATGGCCGTATCATAGGTCCGTATGATCTCATGATTGCCGGGCATGCCCGGAG
CAGAGGGCTGATTGTTGTGACCGGCAATCTGCGGGAATTTCAGCGGGTGGAGGGTCTTCGAACGGAGGACTGGCTGACTG
ACATGGTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8217.39 Da
• Isoelectric Point: 4.9816

>AT76724 WP_010511771.1 NZ_CP021524:1918715-1918939 [Acetobacter ascendens]
MLTRTTLFLSNRSQAVRLPKMVAFGEQVRDVVIVSEGSRRIIAPADAAWDDFFAAPGVDLGERNQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT76724 NZ_CP021524:1918715-1918939 [Acetobacter ascendens]
ATGCTGACCCGTACGACACTCTTTCTATCCAACCGCTCGCAGGCAGTGCGTCTGCCGAAGATGGTCGCCTTCGGGGAACA
GGTGCGTGACGTCGTCATCGTGTCCGAAGGGTCGCGGCGGATCATTGCTCCGGCGGATGCGGCGTGGGATGATTTCTTTG
CGGCTCCCGGGGTGGATCTGGGAGAGCGAAACCAGCCGGCCATGCAGGAACGTGAGGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A094ZE42