Detailed information of TA system TA076714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 548938..549574
• Replicon: chromosome
• Accession: NZ_CP021507
• Organism: Bacillus subtilis subsp. subtilis strain SRCM101441

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: S101441_RS02720
• Protein ID: WP_003156187.1
• Coordinates: 549224..549574 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: S101441_RS02715
• Protein ID: WP_003225183.1
• Coordinates: 548938..549219 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101441_RS02695	545297..545896	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
S101441_RS02700	545991..546356	+	366	WP_014478897.1	holo-ACP synthase	-
S101441_RS02705	546522..547538	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
S101441_RS02710	547653..548822	+	1170	WP_014478898.1	alanine racemase	-
S101441_RS02715	548938..549219	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
S101441_RS02720	549224..549574	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
S101441_RS02725	549690..550514	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
S101441_RS02730	550519..550884	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
S101441_RS02735	550888..551289	+	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
S101441_RS02740	551301..552308	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
S101441_RS02745	552377..552706	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
S101441_RS02750	552703..553185	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
S101441_RS02755	553151..553939	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
S101441_RS02760	553939..554538	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T76714 WP_003156187.1 NZ_CP021507:549224-549574 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T76714 NZ_CP021507:549224-549574 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT76714 WP_003225183.1 NZ_CP021507:548938-549219 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT76714 NZ_CP021507:548938-549219 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32