76713

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1369594..1370510 Replicon chromosome
Accession NZ_CP021505
Organism Bacillus amyloliquefaciens strain SRCM101267

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A7U5AVM4
Locus tag S101267_RS07380 Protein ID WP_013351967.1
Coordinates 1369764..1370510 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag S101267_RS07375 Protein ID WP_003154807.1
Coordinates 1369594..1369764 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S101267_RS07345 1366460..1366858 + 399 WP_013351961.1 hypothetical protein -
S101267_RS07350 1366845..1367042 + 198 WP_013351962.1 XkdX family protein -
S101267_RS07355 1367091..1367852 + 762 WP_013351963.1 hypothetical protein -
S101267_RS07360 1367906..1368169 + 264 WP_013351964.1 hemolysin XhlA family protein -
S101267_RS07365 1368183..1368446 + 264 WP_013351965.1 phage holin -
S101267_RS07370 1368460..1369338 + 879 Protein_1381 N-acetylmuramoyl-L-alanine amidase -
S101267_RS07375 1369594..1369764 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
S101267_RS07380 1369764..1370510 - 747 WP_013351967.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
S101267_RS07385 1370618..1371616 - 999 WP_013351968.1 inorganic phosphate transporter -
S101267_RS07390 1371629..1372246 - 618 WP_003154804.1 DUF47 domain-containing protein -
S101267_RS07395 1372531..1373847 - 1317 WP_013351969.1 amino acid permease -
S101267_RS07400 1374171..1375121 + 951 WP_013351970.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29054.53 Da        Isoelectric Point: 4.6839

>T76713 WP_013351967.1 NZ_CP021505:c1370510-1369764 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T76713 NZ_CP021505:c1370510-1369764 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT76713 WP_003154807.1 NZ_CP021505:c1369764-1369594 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT76713 NZ_CP021505:c1369764-1369594 [Bacillus amyloliquefaciens]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U5AVM4

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References