Detailed information of TA system TA076712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 474679..475316
• Replicon: chromosome
• Accession: NZ_CP021505
• Organism: Bacillus amyloliquefaciens strain SRCM101267

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: S101267_RS02450
• Protein ID: WP_003156187.1
• Coordinates: 474966..475316 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: S101267_RS02445
• Protein ID: WP_003156188.1
• Coordinates: 474679..474960 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101267_RS02425	471045..471644	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
S101267_RS02430	471737..472102	+	366	WP_013351093.1	holo-ACP synthase	-
S101267_RS02435	472267..473274	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
S101267_RS02440	473391..474560	+	1170	WP_013351095.1	alanine racemase	-
S101267_RS02445	474679..474960	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
S101267_RS02450	474966..475316	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
S101267_RS02455	475437..476258	+	822	WP_013351096.1	STAS domain-containing protein	-
S101267_RS02460	476263..476628	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
S101267_RS02465	476631..477032	+	402	WP_013351097.1	anti-sigma regulatory factor	-
S101267_RS02470	477044..478051	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
S101267_RS02475	478115..478444	+	330	WP_013351099.1	anti-sigma factor antagonist	-
S101267_RS02480	478441..478923	+	483	WP_013351100.1	anti-sigma B factor RsbW	-
S101267_RS02485	478889..479677	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
S101267_RS02490	479677..480279	+	603	WP_013351102.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T76712 WP_003156187.1 NZ_CP021505:474966-475316 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T76712 NZ_CP021505:474966-475316 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT76712 WP_003156188.1 NZ_CP021505:474679-474960 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT76712 NZ_CP021505:474679-474960 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5