Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 529847..530483 Replicon chromosome
Accession NZ_CP021500
Organism Bacillus atrophaeus strain SRCM101359

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag S101359_RS02625 Protein ID WP_003156187.1
Coordinates 530133..530483 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag S101359_RS02620 Protein ID WP_003328141.1
Coordinates 529847..530128 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S101359_RS02600 526145..526744 - 600 WP_088116551.1 rhomboid family intramembrane serine protease -
S101359_RS02605 526843..527208 + 366 WP_088116552.1 holo-ACP synthase -
S101359_RS02610 527376..528392 + 1017 WP_088116553.1 outer membrane lipoprotein carrier protein LolA -
S101359_RS02615 528559..529728 + 1170 WP_088116554.1 alanine racemase -
S101359_RS02620 529847..530128 + 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
S101359_RS02625 530133..530483 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
S101359_RS02630 530599..531417 + 819 WP_003328142.1 STAS domain-containing protein -
S101359_RS02635 531422..531787 + 366 WP_010789626.1 RsbT antagonist protein RsbS -
S101359_RS02640 531790..532191 + 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
S101359_RS02645 532203..533210 + 1008 WP_088116555.1 PP2C family protein-serine/threonine phosphatase -
S101359_RS02650 533270..533599 + 330 WP_010789624.1 anti-sigma factor antagonist RsbV -
S101359_RS02655 533596..534078 + 483 WP_003328147.1 anti-sigma B factor RsbW -
S101359_RS02660 534044..534832 + 789 WP_003328149.1 RNA polymerase sigma factor SigB -
S101359_RS02665 534832..535431 + 600 WP_003328150.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T76710 WP_003156187.1 NZ_CP021500:530133-530483 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T76710 NZ_CP021500:530133-530483 [Bacillus atrophaeus]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT76710 WP_003328141.1 NZ_CP021500:529847-530128 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT76710 NZ_CP021500:529847-530128 [Bacillus atrophaeus]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References