Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490100..490737 Replicon chromosome
Accession NZ_CP021495
Organism Bacillus velezensis strain GQJK49

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAGQ_RS02530 Protein ID WP_003156187.1
Coordinates 490387..490737 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAGQ_RS02525 Protein ID WP_003156188.1
Coordinates 490100..490381 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAGQ_RS02505 486465..487064 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
BAGQ_RS02510 487157..487522 + 366 WP_007609580.1 holo-ACP synthase -
BAGQ_RS02515 487687..488694 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
BAGQ_RS02520 488811..489980 + 1170 WP_032873001.1 alanine racemase -
BAGQ_RS02525 490100..490381 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAGQ_RS02530 490387..490737 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAGQ_RS02535 490855..491676 + 822 WP_003156182.1 STAS domain-containing protein -
BAGQ_RS02540 491681..492046 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAGQ_RS02545 492049..492450 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAGQ_RS02550 492462..493469 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BAGQ_RS02555 493533..493862 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BAGQ_RS02560 493859..494341 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BAGQ_RS02565 494307..495095 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
BAGQ_RS02570 495095..495697 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T76704 WP_003156187.1 NZ_CP021495:490387-490737 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T76704 NZ_CP021495:490387-490737 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT76704 WP_003156188.1 NZ_CP021495:490100-490381 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT76704 NZ_CP021495:490100-490381 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References