Detailed information of TA system TA076696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4206200..4207020
• Replicon: chromosome
• Accession: NZ_CP021454
• Organism: Escherichia coli strain H105

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: CCL28_RS21760
• Protein ID: WP_001054379.1
• Coordinates: 4206200..4206457 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: CCL28_RS21765
• Protein ID: WP_000123957.1
• Coordinates: 4206469..4207020 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL28_RS21730	4201747..4202727	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
CCL28_RS21740	4203310..4204416	-	1107	Protein_4011	helicase YjhR	-
CCL28_RS21745	4204479..4205626	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
CCL28_RS21750	4205659..4205823	+	165	Protein_4013	GNAT family N-acetyltransferase	-
CCL28_RS21760	4206200..4206457	+	258	WP_001054379.1	YjhX family toxin	Toxin
CCL28_RS21765	4206469..4207020	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
CCL28_RS21770	4207072..4207818	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
CCL28_RS21775	4207946..4208206	+	261	WP_000077645.1	hypothetical protein	-
CCL28_RS27150	4208244..4208360	+	117	Protein_4018	VOC family protein	-
CCL28_RS21785	4208605..4209726	+	1122	WP_000010833.1	M42 family metallopeptidase	-
CCL28_RS21790	4209723..4210001	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CCL28_RS21795	4210013..4211326	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T76696 WP_001054379.1 NZ_CP021454:4206200-4206457 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T76696 NZ_CP021454:4206200-4206457 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT76696 WP_000123957.1 NZ_CP021454:4206469-4207020 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT76696 NZ_CP021454:4206469-4207020 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030