Detailed information of TA system TA076672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2853645..2855231
• Replicon: chromosome
• Accession: NZ_CP021448
• Organism: Ralstonia solanacearum strain SEPPX05

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: RSSE_RS14090
• Protein ID: WP_087451931.1
• Coordinates: 2853645..2854982 (-)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A223GLT8
• Locus tag: RSSE_RS14095
• Protein ID: WP_028853251.1
• Coordinates: 2854986..2855231 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSSE_RS14065	2849290..2850030	+	741	WP_087451927.1	protocatechuate 3,4-dioxygenase subunit beta	-
RSSE_RS14070	2850027..2850725	+	699	WP_087451928.1	protocatechuate 3,4-dioxygenase subunit alpha	-
RSSE_RS14075	2850906..2851469	+	564	WP_080511083.1	RNA 2',3'-cyclic phosphodiesterase	-
RSSE_RS14080	2851492..2852220	-	729	WP_087451929.1	DsbA family protein	-
RSSE_RS14085	2852446..2853648	-	1203	WP_087451930.1	MFS transporter	-
RSSE_RS14090	2853645..2854982	-	1338	WP_087451931.1	type II toxin-antitoxin system HipA family toxin	Toxin
RSSE_RS14095	2854986..2855231	-	246	WP_028853251.1	helix-turn-helix transcriptional regulator	Antitoxin
RSSE_RS14100	2855335..2856174	-	840	WP_087451932.1	oxaloacetate decarboxylase	-
RSSE_RS14110	2856492..2857130	+	639	WP_087451933.1	7-carboxy-7-deazaguanine synthase	-
RSSE_RS14115	2857198..2857674	+	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
RSSE_RS14120	2857694..2858245	+	552	WP_011001400.1	tRNA adenosine(34) deaminase TadA	-
RSSE_RS14125	2858242..2859168	+	927	WP_028860692.1	muramoyltetrapeptide carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-63)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48767.87 Da
• Isoelectric Point: 7.0609

>T76672 WP_087451931.1 NZ_CP021448:c2854982-2853645 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLKRVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T76672 NZ_CP021448:c2854982-2853645 [Ralstonia solanacearum]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGCCTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACGGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGACCGCATCGACAGCGAGCCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCACGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCACCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGTCGCTGGATGCCGGGCAACGCCTGGATCGCGCGCCTGCCGCAGGAGGACTTCTGCCAGGCCACCGGCACGCCCGC
GCACCGCAAATACGAAAACGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTGCTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTACGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGTTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCAAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8482.80 Da
• Isoelectric Point: 10.4693

>AT76672 WP_028853251.1 NZ_CP021448:c2855231-2854986 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Nucleotide

• Length: 246 bp

>AT76672 NZ_CP021448:c2855231-2854986 [Ralstonia solanacearum]
ATGGAGTCCCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTGCTGCGCATCCTGCACGCGCTCGGCGCGCAGCTGACGCTGCGCACGTCCGCGCCCGCCGCCGCCGCGCCCGCCGAC
TGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A223GLT8