Detailed information of TA system TA076670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 632737..633326
• Replicon: chromosome
• Accession: NZ_CP021448
• Organism: Ralstonia solanacearum strain SEPPX05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: RSSE_RS03135
• Protein ID: WP_087451242.1
• Coordinates: 632737..632991 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q8XYR1
• Locus tag: RSSE_RS03140
• Protein ID: WP_011001638.1
• Coordinates: 632988..633326 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSSE_RS03115	627977..628468	-	492	WP_087451239.1	glycoside hydrolase family protein	-
RSSE_RS03120	628537..629193	-	657	WP_087451240.1	hypothetical protein	-
RSSE_RS03130	629530..632685	-	3156	WP_087451241.1	host specificity protein J	-
RSSE_RS03135	632737..632991	+	255	WP_087451242.1	type II toxin-antitoxin system HicA family toxin	Toxin
RSSE_RS03140	632988..633326	+	339	WP_011001638.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RSSE_RS03145	633396..633998	-	603	WP_087451243.1	tail assembly protein	-
RSSE_RS03150	634002..634712	-	711	WP_087451244.1	C40 family peptidase	-
RSSE_RS03155	634714..635415	-	702	WP_087451245.1	phage minor tail protein L	-
RSSE_RS03160	635412..636008	-	597	WP_087451246.1	tail fiber assembly protein	-
RSSE_RS03165	636014..636691	-	678	WP_087451247.1	phage tail protein	-
RSSE_RS03170	636688..637026	-	339	WP_087451248.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	622694..663523	40829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9458.03 Da
• Isoelectric Point: 10.3121

>T76670 WP_087451242.1 NZ_CP021448:632737-632991 [Ralstonia solanacearum]
MKAKHQKTLELIFSRPTPAGVKWADAVALMRELGAELEEREGSRVAVFLFGQVKVMHRPHPSPDMDKGAVASMRKWFEEN
GVKP

Nucleotide

• Length: 255 bp

>T76670 NZ_CP021448:632737-632991 [Ralstonia solanacearum]
ATGAAAGCCAAACACCAGAAGACGCTGGAGCTGATCTTTTCCCGCCCTACGCCAGCGGGTGTTAAGTGGGCCGATGCCGT
CGCGCTCATGCGAGAACTGGGCGCGGAATTGGAGGAGCGCGAGGGTTCTCGCGTGGCCGTCTTCCTGTTTGGTCAAGTTA
AAGTGATGCATCGTCCACACCCATCGCCCGATATGGACAAGGGCGCTGTGGCCTCGATGCGTAAGTGGTTCGAGGAAAAC
GGAGTCAAGCCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12007.63 Da
• Isoelectric Point: 5.1236

>AT76670 WP_011001638.1 NZ_CP021448:632988-633326 [Ralstonia solanacearum]
MINVMNIGGHKAVIAYDPDIEMFRGEFVGLNGGADFYAADVPGLHREGELSLHVFLEECARRGVEPQKHFSGKFMLRVEG
KVHEAATIAAAAQGVSLNQWAAGVLEQAAEAA

Nucleotide

• Length: 339 bp

>AT76670 NZ_CP021448:632988-633326 [Ralstonia solanacearum]
ATGATCAACGTCATGAACATCGGCGGCCACAAGGCCGTCATCGCCTACGACCCGGATATCGAAATGTTCCGTGGAGAGTT
TGTTGGCCTGAACGGTGGCGCCGATTTCTACGCCGCCGATGTACCCGGCCTGCACCGCGAAGGCGAGTTGTCGCTGCACG
TGTTTCTGGAAGAGTGCGCACGGCGTGGCGTTGAACCGCAAAAGCACTTTTCTGGCAAGTTCATGCTGCGAGTGGAGGGC
AAGGTGCACGAGGCCGCCACCATCGCCGCTGCCGCGCAAGGCGTGAGCCTTAACCAGTGGGCAGCCGGCGTGCTGGAGCA
AGCCGCCGAGGCGGCTTAA

Structures

Antitoxin

• AlphaFold DB: Q8XYR1