76644

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1783872..1784304 Replicon chromosome
Accession NZ_CP021445
Organism Clostridioides difficile strain DSM 104450

Toxin (Protein)


Gene name zeta Uniprot ID R4ZD79
Locus tag CDIF104450_RS08850 Protein ID WP_003423072.1
Coordinates 1783872..1784030 (-) Length 53 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag CDIF104450_RS08855 Protein ID WP_000301765.1
Coordinates 1784032..1784304 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDIF104450_RS08830 (CDIF104450_01758) 1780170..1780736 - 567 WP_009889452.1 MarR family transcriptional regulator -
CDIF104450_RS08835 (CDIF104450_01759) 1781024..1781959 + 936 WP_021366611.1 alpha/beta hydrolase -
CDIF104450_RS08840 (CDIF104450_01760) 1782424..1782552 + 129 Protein_1655 helix-turn-helix domain-containing protein -
CDIF104450_RS08845 (CDIF104450_01762) 1783275..1783820 - 546 WP_021391072.1 AAA family ATPase -
CDIF104450_RS08850 1783872..1784030 - 159 WP_003423072.1 zeta toxin family protein Toxin
CDIF104450_RS08855 (CDIF104450_01763) 1784032..1784304 - 273 WP_000301765.1 antitoxin Antitoxin
CDIF104450_RS08860 (CDIF104450_01764) 1784321..1784536 - 216 WP_002321610.1 peptide-binding protein -
CDIF104450_RS08865 (CDIF104450_01765) 1784628..1785524 - 897 WP_001809760.1 ParA family protein -
CDIF104450_RS08870 1785627..1785887 - 261 Protein_1661 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
CDIF104450_RS08875 (CDIF104450_01766) 1786057..1786794 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
CDIF104450_RS08880 (CDIF104450_01767) 1786841..1787080 - 240 WP_002425012.1 peptide-binding protein -
CDIF104450_RS08885 (CDIF104450_01768) 1787200..1789140 - 1941 Protein_1664 DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) - 1693390..1834317 140927


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-50)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5776.53 Da        Isoelectric Point: 10.3088

>T76644 WP_003423072.1 NZ_CP021445:c1784030-1783872 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR

Download         Length: 159 bp

>T76644 NZ_CP021445:c1784030-1783872 [Clostridioides difficile]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAAAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACGAGTTTGCGAAGAAGATGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT76644 WP_000301765.1 NZ_CP021445:c1784304-1784032 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT76644 NZ_CP021445:c1784304-1784032 [Clostridioides difficile]
ATGGCAGTTACTTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGCTATTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4ZD79

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References