Detailed information of TA system TA076616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 26972..27556
• Replicon: plasmid p11
• Accession: NZ_CP021429
• Organism: Acinetobacter pittii strain HUMV-6483

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: CCK83_RS20095
• Protein ID: WP_000286963.1
• Coordinates: 26972..27274 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CCK83_RS20100
• Protein ID: WP_006582667.1
• Coordinates: 27275..27556 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCK83_RS20070	22132..23457	+	1326	WP_016805832.1	ISNCY family transposase	-
CCK83_RS20075	24043..24825	-	783	WP_006582115.1	hypothetical protein	-
CCK83_RS20085	25322..26487	+	1166	WP_085944217.1	IS3-like element ISAba22 family transposase	-
CCK83_RS20090	26530..26772	-	243	Protein_35	hypothetical protein	-
CCK83_RS20095	26972..27274	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCK83_RS20100	27275..27556	+	282	WP_006582667.1	putative addiction module antidote protein	Antitoxin
CCK83_RS20105	27848..29056	+	1209	WP_006582666.1	IS256 family transposase	-
CCK83_RS20110	29099..31765	+	2667	WP_002055446.1	excinuclease ABC subunit UvrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112604	112604
	-	inside	IScluster/Tn	-	-	22120..38320	16200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T76616 WP_000286963.1 NZ_CP021429:26972-27274 [Acinetobacter pittii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T76616 NZ_CP021429:26972-27274 [Acinetobacter pittii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10139.70 Da
• Isoelectric Point: 8.0018

>AT76616 WP_006582667.1 NZ_CP021429:27275-27556 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLTI

Nucleotide

• Length: 282 bp

>AT76616 NZ_CP021429:27275-27556 [Acinetobacter pittii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAACAATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2