Detailed information of TA system TA076607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 117590..118620
• Replicon: plasmid pDY25-B
• Accession: NZ_CP021406
• Organism: Celeribacter manganoxidans strain DY25

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CBW24_RS16690
• Protein ID: WP_007803220.1
• Coordinates: 118051..118620 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: V9WPQ5
• Locus tag: CBW24_RS16685
• Protein ID: WP_007803221.1
• Coordinates: 117590..118054 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBW24_RS16665	112747..113730	-	984	WP_097374462.1	plasmid partitioning protein RepB	-
CBW24_RS16670	113715..114914	-	1200	WP_097374463.1	plasmid partitioning protein RepA	-
CBW24_RS16675	115257..116182	-	926	Protein_127	recombinase family protein	-
CBW24_RS16680	116373..117440	+	1068	WP_007803223.1	hypothetical protein	-
CBW24_RS16685	117590..118054	+	465	WP_007803221.1	excisionase family DNA-binding protein	Antitoxin
CBW24_RS16690	118051..118620	+	570	WP_007803220.1	PIN domain-containing protein	Toxin
CBW24_RS16700	119227..120861	+	1635	WP_139274568.1	Mu transposase C-terminal domain-containing protein	-
CBW24_RS16705	120863..121744	+	882	WP_093037101.1	TniB family NTP-binding protein	-
CBW24_RS16710	121741..122532	+	792	WP_093037357.1	TniQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..178640	178640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (81-127)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21163.39 Da
• Isoelectric Point: 5.2804

>T76607 WP_007803220.1 NZ_CP021406:118051-118620 [Celeribacter manganoxidans]
MSQYTVLFDANVLYPAPMRDALMQLAVTDLFKAKWTADIHREWIDALLRNEPHRERAALERTRDLMDRATRDCLVTGYEA
LVPALTLPDPDDRHVLAAAIVGRCDAIVTQNLKDFPPAALAPFGIETQHPDDFFRNQLSLAPGLVCSALRKVRARLKNPL
KSVDEYLAILTQQGLVATVADLEQFADLL

Nucleotide

• Length: 570 bp

>T76607 NZ_CP021406:118051-118620 [Celeribacter manganoxidans]
ATGAGCCAGTACACGGTCCTGTTCGATGCGAACGTCCTTTATCCCGCGCCGATGCGCGATGCACTGATGCAGTTGGCGGT
CACAGACCTTTTCAAGGCCAAGTGGACGGCCGATATCCATCGGGAGTGGATTGATGCGCTTCTGCGCAATGAACCTCATC
GGGAGCGCGCCGCGCTGGAACGAACTCGCGACTTGATGGACAGGGCCACACGCGACTGTCTGGTCACCGGCTACGAGGCA
TTGGTGCCGGCCCTAACACTGCCGGACCCTGACGATCGGCACGTTCTGGCGGCGGCAATCGTTGGGCGCTGTGACGCGAT
CGTCACGCAGAACCTGAAGGACTTCCCACCAGCGGCACTTGCGCCCTTTGGCATCGAGACCCAACACCCTGACGACTTTT
TCCGGAACCAACTGTCTCTCGCGCCCGGTCTGGTCTGTTCCGCGCTGCGGAAAGTTCGCGCGCGCCTCAAGAATCCGCTC
AAAAGCGTCGACGAGTATCTCGCGATCCTGACACAGCAGGGGTTGGTCGCCACGGTTGCCGACCTGGAGCAATTCGCCGA
CCTGCTTTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16891.48 Da
• Isoelectric Point: 6.4929

>AT76607 WP_007803221.1 NZ_CP021406:117590-118054 [Celeribacter manganoxidans]
MNMLAHRHLPPTPQDAAIARVSGQALSRFAQARAPLKLRVTDSEQMEPIELPAGAVSLLMEILEAMAAGRGVTIIPENAE
LSTVQAAEVLNVSRPFLIKLLEDGAIPHRKVGKHRRVRMEDVMSYKAAIDTEREAALDQLAADAQEQDMGYRSK

Nucleotide

• Length: 465 bp

>AT76607 NZ_CP021406:117590-118054 [Celeribacter manganoxidans]
ATGAACATGCTGGCACACCGACATCTTCCGCCCACGCCGCAGGACGCAGCGATTGCGCGCGTCTCTGGCCAGGCATTGTC
ACGCTTCGCCCAGGCGCGCGCGCCGTTGAAACTTCGTGTGACGGACTCCGAGCAGATGGAGCCGATCGAGCTTCCTGCGG
GCGCCGTATCGCTGCTCATGGAGATCCTCGAGGCGATGGCAGCGGGGCGGGGGGTCACCATCATCCCCGAGAACGCCGAA
CTCTCGACCGTACAGGCCGCTGAGGTTCTGAACGTCTCGCGTCCGTTCCTGATCAAGCTGCTCGAGGATGGTGCCATACC
GCATCGGAAGGTCGGCAAGCATCGCCGCGTCCGCATGGAAGACGTGATGTCCTACAAGGCCGCGATCGATACCGAGCGTG
AAGCTGCGCTCGATCAACTGGCCGCCGACGCTCAAGAGCAGGACATGGGCTACCGCTCGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9Z9S0